Additional file 14 of Receptor deorphanization in an echinoderm reveals kisspeptin evolution and relationship with SALMFamide neuropeptides

  • Nayeli Escudero Castelán (Creator)
  • Dean C. Semmens (Creator)
  • Luis Alfonso Yañez-Guerra (Creator)
  • Meet Zandawala (Creator)
  • Mario dos Reis (Creator)
  • Susan E. Slade (Creator)
  • James H. Scrivens (University of Warwick) (Creator)
  • Cleidiane G. Zampronio (Creator)
  • Alexandra M. Jones (Creator)
  • Olivier Mirabeau (Creator)
  • Maurice R. Elphick (Creator)



Additional file 14. Comparison of luminescence measurements of CHO-K1 cells transfected with the A. rubens kisspeptin-type receptors ArKPR1-11 at 35 seconds after exposure to candidate peptide ligands (10-5 M). Each bar represents mean values (± S.E.M.) from at least two independent experiments, with each experiment performed in triplicate. ArKP1.1 (green), ArKP1.2 (pink), ArKP2.2 (purple), ‘cocktail’ of L-type SALMFamide precursor derived peptides ArS1.1-7 (blue), ‘cocktail’ of F-type SALMFamide precursor derived peptides ArS2.1-8 (red) and assay media as a negative control (black). Triton X-100 (olive green), which triggers luminescence via receptor-independent mechanisms, was tested as a positive control to check for cell viability. The source data for these experiments are provided in additional file 15.
Date made available2022

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