Additional file 14. Comparison of luminescence measurements of CHO-K1 cells transfected with the A. rubens kisspeptin-type receptors ArKPR1-11 at 35 seconds after exposure to candidate peptide ligands (10-5 M). Each bar represents mean values (± S.E.M.) from at least two independent experiments, with each experiment performed in triplicate. ArKP1.1 (green), ArKP1.2 (pink), ArKP2.2 (purple), ‘cocktail’ of L-type SALMFamide precursor derived peptides ArS1.1-7 (blue), ‘cocktail’ of F-type SALMFamide precursor derived peptides ArS2.1-8 (red) and assay media as a negative control (black). Triton X-100 (olive green), which triggers luminescence via receptor-independent mechanisms, was tested as a positive control to check for cell viability. The source data for these experiments are provided in additional file 15.
|Date made available||2022|