Additional file 19 of Receptor deorphanization in an echinoderm reveals kisspeptin evolution and relationship with SALMFamide neuropeptides

Additional file 19. Concentration-response graphs for A. rubens kisspeptin-type receptors (ArKPR2,4,5,10,11) that were not activated by any of the candidate ligands tested. Key: Green = ArKP1.1, Pink = ArKP1.2, Purple = ArKP2.2; Blue = ‘cocktail’ of neuropeptides derived from the L-type SALMFamide precursor (ArS1.1-7); Red = ‘cocktail’ of neuropeptides derived from the F-type SALMFamide precursor (ArS2.1-8). Each point represents mean values (± S.E.M.) from at least four independent experiments, with each experiment performed in triplicate. Luminescence is expressed as a percentage of the maximal response observed in each experiment. Triton X-100 (olive green), which triggers luminescence via receptor-independent mechanisms, was tested as positive control to check for cell viability and the response to Triton X-100 in each experiment was assigned as 100% luminescence. The source data for these experiments are provided in additional file 15.