Additional file 19 of Receptor deorphanization in an echinoderm reveals kisspeptin evolution and relationship with SALMFamide neuropeptides

  • Nayeli Escudero Castelán (Creator)
  • Dean C. Semmens (Creator)
  • Luis Alfonso Yañez-Guerra (Creator)
  • Meet Zandawala (Creator)
  • Mario dos Reis (Creator)
  • Susan E. Slade (Creator)
  • James H. Scrivens (University of Warwick) (Creator)
  • Cleidiane G. Zampronio (Creator)
  • Alexandra M. Jones (Creator)
  • Olivier Mirabeau (Creator)
  • Maurice R. Elphick (Creator)



Additional file 19. Concentration-response graphs for A. rubens kisspeptin-type receptors (ArKPR2,4,5,10,11) that were not activated by any of the candidate ligands tested. Key: Green = ArKP1.1, Pink = ArKP1.2, Purple = ArKP2.2; Blue = ‘cocktail’ of neuropeptides derived from the L-type SALMFamide precursor (ArS1.1-7); Red = ‘cocktail’ of neuropeptides derived from the F-type SALMFamide precursor (ArS2.1-8). Each point represents mean values (± S.E.M.) from at least four independent experiments, with each experiment performed in triplicate. Luminescence is expressed as a percentage of the maximal response observed in each experiment. Triton X-100 (olive green), which triggers luminescence via receptor-independent mechanisms, was tested as positive control to check for cell viability and the response to Triton X-100 in each experiment was assigned as 100% luminescence. The source data for these experiments are provided in additional file 15.
Date made available2022

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