Additional file 4. Phylogenetic analysis of bilaterian kisspeptin-type receptors, including expanded receptor families in the starfish A. rubens (ArubKPR1-11), other echinoderms, and Branchiostoma floridae. The phylogenetic tree was constructed using the maximum-likelihood method, LG+G4 amino-acid substitution model, and rooted with galanin/allatostatin-A-type receptors as an outgroup. Boostrap support for each node is stated according to SH-aLRT/UFBoot methods. The scale bar indicates amino acid substitutions per site. Species names are as follows: Apla, Acanthaster planci; Ajap, Apostichopus japonicus; Arub, Asterias rubens, Anjap Annessia japonica; Bbel, Branchiostoma belcheri; Bflo B. floridae; Ctel, Capitella teleta; Cgig, Crassostrea gigas; Hsap, Homo sapines; Lcha, Latimeria chalumnae; Locu, Lepisosteus oculatus; Lgig, Lottia gigantea; Mmus, Mus musculus; Pdum, Platynereis dumerilii; Pbiv, Python bivittatus; Skow, Saccoglossus kowalevskii; Spur, Strongylocentrotus purpuratus; Xlae Xenopus laevis; Xtro, X. tropicalis. Accession numbers for the sequences of the receptors included in this tree are listed in additional file 3.
|Date made available||2022|