Additional file 8 of Receptor deorphanization in an echinoderm reveals kisspeptin evolution and relationship with SALMFamide neuropeptides

Additional file 8. CLuster Analysis of Sequences (CLANS) of A. rubens kisspeptin-type receptors (ArKPR1-11) and other kisspeptin-type receptors from bilaterian taxa. BLOSUM62 cluster map of kisspeptin-type receptors and the closely related galanin/allatostatin-A-type receptors. Nodes are labelled with taxon-specific shapes and colors, as shown in the key. Connections represent BLAST relationships with a P value > 1e-40. Galanin/allatostatin-A-type receptors are enclosed within the dashed line. Names shown in dark blue with an associated symbol containing a blue dot are the receptors for which neuropeptide ligands have been identified experimentally in this study or others. Names shown in black with an associated symbol containing a black dot are A. rubens kisspeptin-type receptors for which neuropeptide ligands have yet to be identified. The accession numbers for the receptors shown in this figure are provided in additional file 3.