Mohammad Dadashipour

Mohammad Dadashipour

Dr

Accepting PhD Students

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Personal profile

Summary of Research Interests

In one word:

Enzymes

 

In more words:

Enzyme screening and discovery

Enzyme/ protein engineering and directed evolution

Enzyme/ protein biotechnology and applications

Enzyme biochemistry

Applied enzymology

Biocatalysis

Industrial/ white biotechnology

Upstream and downstream processes (USP & DSP)

Interested in biologically active compounds and naturally occuring proteins and enzymes with medical properties

 

In details:

I have been working on biotechnology, biochemistry, engineering and application of enzymes and proteins for the last 20 years:

  • Both upstream and downstream of proteins and enzymes including:
  1. Enzyme screening and enzyme assays among microorganisms, plants and animals (and metagenome) for diagnosis and biocatalysis
  2. Enzyme and protein production employing native microbial isolates and diverse heterologous recombinant gene expressions (prokaryotic systems like E. coli, its cell-free system and Burkholderia cenocepacia as well as eukaryotic systems like Pichia pastoris, Wheat-germ cell-free and LEXSY)
  3. Enzyme/ protein purifications from the various microbial, plant, and animal (arthropods) sources (native and recombinant) using various chromatography systems
  4. Enzyme characterizations including substrate spectrum and biochemical kinetics
  5. Secondary structure analysis of wild-type versus mutant enzymes and proteins (using FT-IR, CD and Fluorescence spectroscopy)

 

  • Engineering of various enzymes and proteins-Examples are mutagenesis and directed evolution of:
  1. HNLs have higher in vivo solubility in E. coli and for biocatalysis
  2. SerDHs for diagnosis/ as a biomarker
  3. Lipocalins in a glycoengineering project for vaccine development and diagnostic kit preparation

 

  • Biocatalytic reactions using enzymes for the synthesis of pharmaceutical building blocks (main focus on HNLs and sugar-metabolising enzymes)

 

  • Phosphorylated sugar nucleotide-metabolising enzyme from a hyperthermophilic microorganism, Sulfolobus tokodaii and discovery of a unique isomerase enzyme with high biotechnology potential- These enzymes make an end product in each pathway which is a substrate for glycosyltransferases (GTs). These enzymes can transfer the above product onto proteins: a connection with personalised medicine. Proteins with glycans on their structure behave different...

 

PhD and Research Opportunities

Students:

Motivated national and international students having a plan to study in MSc or self-funded PhD applicants (and those who have a plan to apply for PhD scholarship) are strongly couraged to visit the links below and then contact me. 

Note: Please make sure that you are eligible to apply for any of the schemes listed below.

MSc/ postgraduate courses: https://www.tees.ac.uk/postgraduate_courses/biosciences/

PhD and research courses: https://www.tees.ac.uk/sections/research/students_prospective.cfm; 

https://www.tees.ac.uk/sections/postgrad/pg_scholarships.cfm

https://www.findaphd.com/phds/latest/

Funding and student loans: https://www.tees.ac.uk/sections/postgrad/pg_funding.cfm; https://www.gov.uk/doctoral-loan

 

Post-Docs:

If you are interested in applying for Marie Curie Fellowship or similar funding schemes feel free to contact me for primary discussions and application preparation. 

https://marie-sklodowska-curie-actions.ec.europa.eu/actions/postdoctoral-fellowships

https://www.youtube.com/watch?v=NWoNpWUmzsg

https://www.youtube.com/watch?v=kh-0lEgZ2_M