A comparison of labeling and label-free mass spectrometry-based proteomics approaches

Vibhuti J. Patel, Konstantinos Thalassinos, Susan E. Slade, Joanne B. Connolly, Andrew Crombie, J. Colin Murrell, James Scrivens

Research output: Contribution to journalArticleResearchpeer-review

149 Citations (Scopus)

Abstract

The proteome of the recently discovered bacterium Methylocella silvestris has been characterized using three profiling and comparative proteomics approaches. The organism has been grown on two different substrates enabling variations in protein expression to be identified. The results obtained using the experimental approaches have been compared with respect to number of proteins identified, confidence in identification, sequence coverage and agreement of regulated proteins. The sample preparation, instrumental time and sample loading requirements of the differing experiments are compared and discussed. A preliminary screen of the protein regulation results for biological significance has also been performed.

Original languageEnglish
Pages (from-to)3752-3759
Number of pages8
JournalJournal of Proteome Research
Volume8
Issue number7
DOIs
Publication statusPublished - 6 Jul 2009

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Proteomics
Labeling
Mass spectrometry
Labels
Mass Spectrometry
Proteins
Proteome
Bacteria
Substrates
Experiments

Cite this

Patel, V. J., Thalassinos, K., Slade, S. E., Connolly, J. B., Crombie, A., Murrell, J. C., & Scrivens, J. (2009). A comparison of labeling and label-free mass spectrometry-based proteomics approaches. Journal of Proteome Research, 8(7), 3752-3759. https://doi.org/10.1021/pr900080y
Patel, Vibhuti J. ; Thalassinos, Konstantinos ; Slade, Susan E. ; Connolly, Joanne B. ; Crombie, Andrew ; Murrell, J. Colin ; Scrivens, James . / A comparison of labeling and label-free mass spectrometry-based proteomics approaches. In: Journal of Proteome Research. 2009 ; Vol. 8, No. 7. pp. 3752-3759.
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Patel, VJ, Thalassinos, K, Slade, SE, Connolly, JB, Crombie, A, Murrell, JC & Scrivens, J 2009, 'A comparison of labeling and label-free mass spectrometry-based proteomics approaches', Journal of Proteome Research, vol. 8, no. 7, pp. 3752-3759. https://doi.org/10.1021/pr900080y

A comparison of labeling and label-free mass spectrometry-based proteomics approaches. / Patel, Vibhuti J.; Thalassinos, Konstantinos; Slade, Susan E.; Connolly, Joanne B.; Crombie, Andrew; Murrell, J. Colin; Scrivens, James .

In: Journal of Proteome Research, Vol. 8, No. 7, 06.07.2009, p. 3752-3759.

Research output: Contribution to journalArticleResearchpeer-review

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Patel VJ, Thalassinos K, Slade SE, Connolly JB, Crombie A, Murrell JC et al. A comparison of labeling and label-free mass spectrometry-based proteomics approaches. Journal of Proteome Research. 2009 Jul 6;8(7):3752-3759. https://doi.org/10.1021/pr900080y