A new family of cell surface located purine transporters in Microsporidia and related fungal endoparasites

Peter Major, Kacper M Sendra, Paul Dean, Tom A Williams, Andrew K Watson, David T Thwaites, T Martin Embley, Robert P Hirt

Research output: Contribution to journalArticle

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Abstract

Plasma membrane-located transport proteins are key adaptations for obligate intracellular Microsporidia parasites, because they can use them to steal host metabolites the parasites need to grow and replicate. However, despite their importance, the functions and substrate specificities of most Microsporidia transporters are unknown. Here we provide functional data for a family of transporters conserved in all microsporidian genomes and also in the genomes of related endoparasites. The universal retention among otherwise highly reduced genomes indicates an important role for these transporters for intracellular parasites. Using Trachipleistophora hominis, a Microsporidia isolated from an HIV/AIDS patient, as our experimental model, we show that the proteins are ATP and GTP transporters located on the surface of parasites during their intracellular growth and replication. Our work identifies a new route for the acquisition of essential energy and nucleotides for a major group of intracellular parasites that infect most animal species including humans.
Original languageEnglish
JournaleLife
Volume8
DOIs
Publication statusPublished - 29 Jul 2019

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Microsporidia
Parasites
Genes
Genome
Membrane Transport Proteins
Cell membranes
Metabolites
Substrate Specificity
Guanosine Triphosphate
Carrier Proteins
Acquired Immunodeficiency Syndrome
Animals
Theoretical Models
Nucleotides
Adenosine Triphosphate
Cell Membrane
purine
HIV
Substrates
Growth

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Major, P., Sendra, K. M., Dean, P., Williams, T. A., Watson, A. K., Thwaites, D. T., ... Hirt, R. P. (2019). A new family of cell surface located purine transporters in Microsporidia and related fungal endoparasites. eLife, 8. https://doi.org/10.7554/elife.47037
Major, Peter ; Sendra, Kacper M ; Dean, Paul ; Williams, Tom A ; Watson, Andrew K ; Thwaites, David T ; Embley, T Martin ; Hirt, Robert P. / A new family of cell surface located purine transporters in Microsporidia and related fungal endoparasites. In: eLife. 2019 ; Vol. 8.
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Major, P, Sendra, KM, Dean, P, Williams, TA, Watson, AK, Thwaites, DT, Embley, TM & Hirt, RP 2019, 'A new family of cell surface located purine transporters in Microsporidia and related fungal endoparasites', eLife, vol. 8. https://doi.org/10.7554/elife.47037

A new family of cell surface located purine transporters in Microsporidia and related fungal endoparasites. / Major, Peter; Sendra, Kacper M; Dean, Paul; Williams, Tom A; Watson, Andrew K; Thwaites, David T; Embley, T Martin; Hirt, Robert P.

In: eLife, Vol. 8, 29.07.2019.

Research output: Contribution to journalArticle

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