A novel method for load bounds identification for uncertain structures is proposed in the frequency domain. The uncertain parameters are assumed to locate in their intervals and only their bounds rather than their precise information are needed. To quantitatively describe the effect of the interval uncertainty on the load identification in the frequency ranges, the interval extension is then introduced in the frequency response function (FRF)-based least squares approach. Therefore, the load bounds are determined through the summation of the two separate parts including the midpoint part and the perturbed part of the load. The midpoint part is computed by using the Moore–Penrose pseudo-inversion and the perturbed part is transformed into the first derivatives of the midpoint load with respect to the uncertain parameters by applying the truncated total least squares (TTLS). Two numerical examples are investigated to validate that the proposed method is very effective to predict the load bounds for the uncertain structure in frequency domain.