A novel semi-automated direct lysis method for DNA recovery from live and spent 9mm ammunition.

Zuhaib Subhani, Kiera Coleman, David Moore, Tim Clayton, Melanie Brown

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Recovery of cellular material and DNA from ammunition is a potentially valuable process capable of providing probative evidence of criminal use of firearms. However, DNA profiling success rates on ammunition are low, and consequently much of the ammunition recovered from crime scenes is never submitted for DNA analysis. There is also a common assumption that DNA from fired ammunition is likely to have deteriorated following discharge. In this study, DNA recovery and subsequent STR profiling was conducted on live and spent 9mm ammunition, handled by known donors prior to loading. Two methods were compared; the commonly-used double swabbing technique and a novel semi-automated direct lysis method using AutoLys tubes (Hamilton). The direct lysis method involves placing 9mm cartridges into an AutoLys tube and submerging the cartridge in lysis buffer prior to purification. Lysate was recovered by centrifugation facilitated by the AutoLys tube design. It was found that the direct lysis method recovered significantly more DNA and yielded correspondingly improved STR profiles than the double swabbing technique. It was also shown that DNA could be recovered and profiled using the direct lysis method on both live and spent 9mm cartridges. These results demonstrate that DNA suitable for STR analysis can be recovered from spent ammunition with only slightly reduced yields compared to live ammunition. In many cases the last handler of the ammunition was a major contributor to the recovered DNA. It was also found that the ammunition subjected to the direct lysis method did not have any effects on the ballistic markings imparted on the cartridge during the firing process. This shows the compatibility of the direct lysis method with other traditional ammunition examinations.
Original languageEnglish
Number of pages4
JournalForensic Science International: Genetics Supplement Series
Early online date1 Oct 2019
DOIs
Publication statusE-pub ahead of print - 1 Oct 2019

Fingerprint

DNA
DNA Fingerprinting
Firearms
Crime
Centrifugation
Buffers
Tissue Donors

Cite this

@article{effda7f52c1542f2b93694179b659a01,
title = "A novel semi-automated direct lysis method for DNA recovery from live and spent 9mm ammunition.",
abstract = "Recovery of cellular material and DNA from ammunition is a potentially valuable process capable of providing probative evidence of criminal use of firearms. However, DNA profiling success rates on ammunition are low, and consequently much of the ammunition recovered from crime scenes is never submitted for DNA analysis. There is also a common assumption that DNA from fired ammunition is likely to have deteriorated following discharge. In this study, DNA recovery and subsequent STR profiling was conducted on live and spent 9mm ammunition, handled by known donors prior to loading. Two methods were compared; the commonly-used double swabbing technique and a novel semi-automated direct lysis method using AutoLys tubes (Hamilton). The direct lysis method involves placing 9mm cartridges into an AutoLys tube and submerging the cartridge in lysis buffer prior to purification. Lysate was recovered by centrifugation facilitated by the AutoLys tube design. It was found that the direct lysis method recovered significantly more DNA and yielded correspondingly improved STR profiles than the double swabbing technique. It was also shown that DNA could be recovered and profiled using the direct lysis method on both live and spent 9mm cartridges. These results demonstrate that DNA suitable for STR analysis can be recovered from spent ammunition with only slightly reduced yields compared to live ammunition. In many cases the last handler of the ammunition was a major contributor to the recovered DNA. It was also found that the ammunition subjected to the direct lysis method did not have any effects on the ballistic markings imparted on the cartridge during the firing process. This shows the compatibility of the direct lysis method with other traditional ammunition examinations.",
author = "Zuhaib Subhani and Kiera Coleman and David Moore and Tim Clayton and Melanie Brown",
year = "2019",
month = "10",
day = "1",
doi = "10.1016/j.fsigss.2019.09.120",
language = "English",
journal = "Forensic Science International: Genetics Supplement Series",
issn = "1875-1768",
publisher = "Elsevier Ireland Ltd",

}

A novel semi-automated direct lysis method for DNA recovery from live and spent 9mm ammunition. / Subhani, Zuhaib; Coleman, Kiera; Moore, David; Clayton, Tim; Brown, Melanie.

In: Forensic Science International: Genetics Supplement Series, 01.10.2019.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - A novel semi-automated direct lysis method for DNA recovery from live and spent 9mm ammunition.

AU - Subhani, Zuhaib

AU - Coleman, Kiera

AU - Moore, David

AU - Clayton, Tim

AU - Brown, Melanie

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Recovery of cellular material and DNA from ammunition is a potentially valuable process capable of providing probative evidence of criminal use of firearms. However, DNA profiling success rates on ammunition are low, and consequently much of the ammunition recovered from crime scenes is never submitted for DNA analysis. There is also a common assumption that DNA from fired ammunition is likely to have deteriorated following discharge. In this study, DNA recovery and subsequent STR profiling was conducted on live and spent 9mm ammunition, handled by known donors prior to loading. Two methods were compared; the commonly-used double swabbing technique and a novel semi-automated direct lysis method using AutoLys tubes (Hamilton). The direct lysis method involves placing 9mm cartridges into an AutoLys tube and submerging the cartridge in lysis buffer prior to purification. Lysate was recovered by centrifugation facilitated by the AutoLys tube design. It was found that the direct lysis method recovered significantly more DNA and yielded correspondingly improved STR profiles than the double swabbing technique. It was also shown that DNA could be recovered and profiled using the direct lysis method on both live and spent 9mm cartridges. These results demonstrate that DNA suitable for STR analysis can be recovered from spent ammunition with only slightly reduced yields compared to live ammunition. In many cases the last handler of the ammunition was a major contributor to the recovered DNA. It was also found that the ammunition subjected to the direct lysis method did not have any effects on the ballistic markings imparted on the cartridge during the firing process. This shows the compatibility of the direct lysis method with other traditional ammunition examinations.

AB - Recovery of cellular material and DNA from ammunition is a potentially valuable process capable of providing probative evidence of criminal use of firearms. However, DNA profiling success rates on ammunition are low, and consequently much of the ammunition recovered from crime scenes is never submitted for DNA analysis. There is also a common assumption that DNA from fired ammunition is likely to have deteriorated following discharge. In this study, DNA recovery and subsequent STR profiling was conducted on live and spent 9mm ammunition, handled by known donors prior to loading. Two methods were compared; the commonly-used double swabbing technique and a novel semi-automated direct lysis method using AutoLys tubes (Hamilton). The direct lysis method involves placing 9mm cartridges into an AutoLys tube and submerging the cartridge in lysis buffer prior to purification. Lysate was recovered by centrifugation facilitated by the AutoLys tube design. It was found that the direct lysis method recovered significantly more DNA and yielded correspondingly improved STR profiles than the double swabbing technique. It was also shown that DNA could be recovered and profiled using the direct lysis method on both live and spent 9mm cartridges. These results demonstrate that DNA suitable for STR analysis can be recovered from spent ammunition with only slightly reduced yields compared to live ammunition. In many cases the last handler of the ammunition was a major contributor to the recovered DNA. It was also found that the ammunition subjected to the direct lysis method did not have any effects on the ballistic markings imparted on the cartridge during the firing process. This shows the compatibility of the direct lysis method with other traditional ammunition examinations.

U2 - 10.1016/j.fsigss.2019.09.120

DO - 10.1016/j.fsigss.2019.09.120

M3 - Article

JO - Forensic Science International: Genetics Supplement Series

JF - Forensic Science International: Genetics Supplement Series

SN - 1875-1768

ER -