Acetyltransferase activity in Candida tropicalis and characteristics of CAT disruption recombinant

Jian Zhang, Hong Gao, Chun Li, Zhu'an Cao

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2 Citations (Scopus)


A rapid method of partially purifying carnitine acetyltransferase (CAT) from Candida tropicalis and determination of its enzyme activity was established. The characteristics of CAT-disruption recombinant of Candida tropicalis were also studied. It was confirmed that the specific activity of CAT of this recombinant was approximately half of that of its corresponding wild type. Therefore, the β-oxidation pathway of dicarboxylic acid was effectively suppressed. Correspondingly, the dicarboxylic acid productivity of this recombinant was increased by 11% and its alkane conversion rate was also increased by 12% in average.

Original languageChinese (Simplified)
Pages (from-to)511-517
Number of pages7
JournalHuagong Xuebao/Journal of Chemical Industry and Engineering (China)
Issue number3
Publication statusPublished - 1 Mar 2005

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