Application of a novel peptide-mediated phage assay for the detection of viable Mycobacterium avium subsp. paratuberculosis to bovine bulk tank milk and feces samples

Antonio Foddai; Samuel  Strain; Robert  Whitlock; Christopher T.  Elliott; Irene Grant

doi:10.1128/JCM.00429-11

Application of a novel peptide-mediated phage assay for the detection of viable Mycobacterium avium subsp. paratuberculosis to bovine bulk tank milk and feces samples

Antonio Foddai, Samuel Strain, Robert Whitlock, Christopher T. Elliott, Irene Grant

Research output: Contribution to journal › Article › peer-review

Abstract

Naturally contaminated bovine bulk tank milk (n = 44) and feces (n = 39) were tested for the presence of viable Mycobacterium avium subsp. paratuberculosis by a novel peptide-mediated magnetic separation-phage (PMS-phage) assay. Counts of viable M. avium subsp. paratuberculosis cells ranging from 1 to 110 PFU/50 ml of milk and 6 to 41,111 PFU/g of feces were indicated by the PMS-phage assay.

Original language	English
Pages (from-to)	2017-2019
Journal	Journal of Clinical Microbiology
Volume	45
Issue number	9
DOIs	https://doi.org/10.1128/JCM.00429-11
Publication status	Published - 1 May 2011
Externally published	Yes

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10.1128/JCM.00429-11

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title = "Application of a novel peptide-mediated phage assay for the detection of viable Mycobacterium avium subsp. paratuberculosis to bovine bulk tank milk and feces samples",

abstract = "Naturally contaminated bovine bulk tank milk (n = 44) and feces (n = 39) were tested for the presence of viable Mycobacterium avium subsp. paratuberculosis by a novel peptide-mediated magnetic separation-phage (PMS-phage) assay. Counts of viable M. avium subsp. paratuberculosis cells ranging from 1 to 110 PFU/50 ml of milk and 6 to 41,111 PFU/g of feces were indicated by the PMS-phage assay.",

author = "Antonio Foddai and Samuel Strain and Robert Whitlock and Elliott, {Christopher T.} and Irene Grant",

year = "2011",

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language = "English",

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Application of a novel peptide-mediated phage assay for the detection of viable Mycobacterium avium subsp. paratuberculosis to bovine bulk tank milk and feces samples. / Foddai, Antonio; Strain, Samuel ; Whitlock, Robert et al.
In: Journal of Clinical Microbiology, Vol. 45, No. 9, 01.05.2011, p. 2017-2019.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Application of a novel peptide-mediated phage assay for the detection of viable Mycobacterium avium subsp. paratuberculosis to bovine bulk tank milk and feces samples

AU - Foddai, Antonio

AU - Strain, Samuel

AU - Whitlock, Robert

AU - Elliott, Christopher T.

AU - Grant, Irene

PY - 2011/5/1

Y1 - 2011/5/1

N2 - Naturally contaminated bovine bulk tank milk (n = 44) and feces (n = 39) were tested for the presence of viable Mycobacterium avium subsp. paratuberculosis by a novel peptide-mediated magnetic separation-phage (PMS-phage) assay. Counts of viable M. avium subsp. paratuberculosis cells ranging from 1 to 110 PFU/50 ml of milk and 6 to 41,111 PFU/g of feces were indicated by the PMS-phage assay.

AB - Naturally contaminated bovine bulk tank milk (n = 44) and feces (n = 39) were tested for the presence of viable Mycobacterium avium subsp. paratuberculosis by a novel peptide-mediated magnetic separation-phage (PMS-phage) assay. Counts of viable M. avium subsp. paratuberculosis cells ranging from 1 to 110 PFU/50 ml of milk and 6 to 41,111 PFU/g of feces were indicated by the PMS-phage assay.

U2 - 10.1128/JCM.00429-11

DO - 10.1128/JCM.00429-11

M3 - Article

SN - 0095-1137

VL - 45

SP - 2017

EP - 2019

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 9

ER -

Application of a novel peptide-mediated phage assay for the detection of viable Mycobacterium avium subsp. paratuberculosis to bovine bulk tank milk and feces samples

Abstract

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