Application of a novel peptide-mediated phage assay for the detection of viable Mycobacterium avium subsp. paratuberculosis to bovine bulk tank milk and feces samples

Antonio Foddai; Samuel  Strain; Robert  Whitlock; Christopher T.  Elliott; Irene Grant

doi:10.1128/JCM.00429-11

Application of a novel peptide-mediated phage assay for the detection of viable Mycobacterium avium subsp. paratuberculosis to bovine bulk tank milk and feces samples

Antonio Foddai
, Samuel Strain
, Robert Whitlock
, Christopher T. Elliott
, Irene Grant

Research output: Contribution to journal › Article › peer-review

Abstract

Naturally contaminated bovine bulk tank milk (n = 44) and feces (n = 39) were tested for the presence of viable Mycobacterium avium subsp. paratuberculosis by a novel peptide-mediated magnetic separation-phage (PMS-phage) assay. Counts of viable M. avium subsp. paratuberculosis cells ranging from 1 to 110 PFU/50 ml of milk and 6 to 41,111 PFU/g of feces were indicated by the PMS-phage assay.

Original language	English
Pages (from-to)	2017-2019
Journal	Journal of Clinical Microbiology
Volume	45
Issue number	9
DOIs	https://doi.org/10.1128/JCM.00429-11
Publication status	Published - 1 May 2011
Externally published	Yes

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10.1128/JCM.00429-11

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title = "Application of a novel peptide-mediated phage assay for the detection of viable Mycobacterium avium subsp. paratuberculosis to bovine bulk tank milk and feces samples",

abstract = "Naturally contaminated bovine bulk tank milk (n = 44) and feces (n = 39) were tested for the presence of viable Mycobacterium avium subsp. paratuberculosis by a novel peptide-mediated magnetic separation-phage (PMS-phage) assay. Counts of viable M. avium subsp. paratuberculosis cells ranging from 1 to 110 PFU/50 ml of milk and 6 to 41,111 PFU/g of feces were indicated by the PMS-phage assay.",

author = "Antonio Foddai and Samuel Strain and Robert Whitlock and Elliott, \{Christopher T.\} and Irene Grant",

year = "2011",

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doi = "10.1128/JCM.00429-11",

language = "English",

volume = "45",

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publisher = "American Society for Microbiology",

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Application of a novel peptide-mediated phage assay for the detection of viable Mycobacterium avium subsp. paratuberculosis to bovine bulk tank milk and feces samples. / Foddai, Antonio; Strain, Samuel ; Whitlock, Robert et al.
In: Journal of Clinical Microbiology, Vol. 45, No. 9, 01.05.2011, p. 2017-2019.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Application of a novel peptide-mediated phage assay for the detection of viable Mycobacterium avium subsp. paratuberculosis to bovine bulk tank milk and feces samples

AU - Foddai, Antonio

AU - Strain, Samuel

AU - Whitlock, Robert

AU - Elliott, Christopher T.

AU - Grant, Irene

PY - 2011/5/1

Y1 - 2011/5/1

N2 - Naturally contaminated bovine bulk tank milk (n = 44) and feces (n = 39) were tested for the presence of viable Mycobacterium avium subsp. paratuberculosis by a novel peptide-mediated magnetic separation-phage (PMS-phage) assay. Counts of viable M. avium subsp. paratuberculosis cells ranging from 1 to 110 PFU/50 ml of milk and 6 to 41,111 PFU/g of feces were indicated by the PMS-phage assay.

AB - Naturally contaminated bovine bulk tank milk (n = 44) and feces (n = 39) were tested for the presence of viable Mycobacterium avium subsp. paratuberculosis by a novel peptide-mediated magnetic separation-phage (PMS-phage) assay. Counts of viable M. avium subsp. paratuberculosis cells ranging from 1 to 110 PFU/50 ml of milk and 6 to 41,111 PFU/g of feces were indicated by the PMS-phage assay.

U2 - 10.1128/JCM.00429-11

DO - 10.1128/JCM.00429-11

M3 - Article

SN - 0095-1137

VL - 45

SP - 2017

EP - 2019

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 9

ER -

Application of a novel peptide-mediated phage assay for the detection of viable Mycobacterium avium subsp. paratuberculosis to bovine bulk tank milk and feces samples

Abstract

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