Assembly of a polymer lab-on-chip device for impedimetric measurements of D-dimers in whole blood

A. Ohlander; C. Strohhofer; K. Bock; S. M. Scott; Z. Ali; Peter Musil; Jan Kyselovic

doi:10.1109/ECTC.2010.5490824

Assembly of a polymer lab-on-chip device for impedimetric measurements of D-dimers in whole blood

A. Ohlander, C. Strohhofer, K. Bock, S. M. Scott, Z. Ali, Peter Musil, Jan Kyselovic

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Abstract

This paper reports the development and characterisation of an assembly technology for a polymer lab-on-chip. The system consists of a 150 μm deep hot embossed microfluidic channel in polycarbonate and Au electrodes fabricated separately by photolithography on polyethylenenaphthalate. The system is designed for impedimetric immunoassay detection in whole blood. Electrode layer and microfluidic substrate are joined by means of a 50 μm thick double-sided medical grade adhesive tape, adjusted with an optical alignment system. The bond proved to be liquid tight at room temperature. An alignment accuracy of 34 μm (+/- 19 μm) evaluated over a set of 23 samples, was achieved. The effect of alignment accuracy of the intermediate adhesive film on whole blood flow properties in the device is studied. Already an alignment error of 70 μm increases the flushing out time of whole blood by approximately 20%.

Original language	English
Pages	1004-1009
DOIs	https://doi.org/10.1109/ECTC.2010.5490824
Publication status	Published - Jun 2010
Event	IEEE 60th Electronic Components and Technology Conference - Las Vegas, United States Duration: 1 Jun 2010 → 4 Jun 2010

Conference

Conference	IEEE 60th Electronic Components and Technology Conference
Abbreviated title	ECTC 2010
Country/Territory	United States
Period	1/06/10 → 4/06/10

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10.1109/ECTC.2010.5490824

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http://ieeexplore.ieee.org/document/5490824/

Cite this

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title = "Assembly of a polymer lab-on-chip device for impedimetric measurements of D-dimers in whole blood",

abstract = "This paper reports the development and characterisation of an assembly technology for a polymer lab-on-chip. The system consists of a 150 μm deep hot embossed microfluidic channel in polycarbonate and Au electrodes fabricated separately by photolithography on polyethylenenaphthalate. The system is designed for impedimetric immunoassay detection in whole blood. Electrode layer and microfluidic substrate are joined by means of a 50 μm thick double-sided medical grade adhesive tape, adjusted with an optical alignment system. The bond proved to be liquid tight at room temperature. An alignment accuracy of 34 μm (+/- 19 μm) evaluated over a set of 23 samples, was achieved. The effect of alignment accuracy of the intermediate adhesive film on whole blood flow properties in the device is studied. Already an alignment error of 70 μm increases the flushing out time of whole blood by approximately 20%.",

author = "A. Ohlander and C. Strohhofer and K. Bock and Scott, {S. M.} and Z. Ali and Peter Musil and Jan Kyselovic",

year = "2010",

month = jun,

doi = "10.1109/ECTC.2010.5490824",

language = "English",

pages = "1004--1009",

note = "IEEE 60th Electronic Components and Technology Conference, ECTC 2010 ; Conference date: 01-06-2010 Through 04-06-2010",

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T1 - Assembly of a polymer lab-on-chip device for impedimetric measurements of D-dimers in whole blood

AU - Ohlander, A.

AU - Strohhofer, C.

AU - Bock, K.

AU - Scott, S. M.

AU - Ali, Z.

AU - Musil, Peter

AU - Kyselovic, Jan

PY - 2010/6

Y1 - 2010/6

N2 - This paper reports the development and characterisation of an assembly technology for a polymer lab-on-chip. The system consists of a 150 μm deep hot embossed microfluidic channel in polycarbonate and Au electrodes fabricated separately by photolithography on polyethylenenaphthalate. The system is designed for impedimetric immunoassay detection in whole blood. Electrode layer and microfluidic substrate are joined by means of a 50 μm thick double-sided medical grade adhesive tape, adjusted with an optical alignment system. The bond proved to be liquid tight at room temperature. An alignment accuracy of 34 μm (+/- 19 μm) evaluated over a set of 23 samples, was achieved. The effect of alignment accuracy of the intermediate adhesive film on whole blood flow properties in the device is studied. Already an alignment error of 70 μm increases the flushing out time of whole blood by approximately 20%.

AB - This paper reports the development and characterisation of an assembly technology for a polymer lab-on-chip. The system consists of a 150 μm deep hot embossed microfluidic channel in polycarbonate and Au electrodes fabricated separately by photolithography on polyethylenenaphthalate. The system is designed for impedimetric immunoassay detection in whole blood. Electrode layer and microfluidic substrate are joined by means of a 50 μm thick double-sided medical grade adhesive tape, adjusted with an optical alignment system. The bond proved to be liquid tight at room temperature. An alignment accuracy of 34 μm (+/- 19 μm) evaluated over a set of 23 samples, was achieved. The effect of alignment accuracy of the intermediate adhesive film on whole blood flow properties in the device is studied. Already an alignment error of 70 μm increases the flushing out time of whole blood by approximately 20%.

U2 - 10.1109/ECTC.2010.5490824

DO - 10.1109/ECTC.2010.5490824

M3 - Paper

SP - 1004

EP - 1009

T2 - IEEE 60th Electronic Components and Technology Conference

Y2 - 1 June 2010 through 4 June 2010

ER -

Assembly of a polymer lab-on-chip device for impedimetric measurements of D-dimers in whole blood

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