Atrazine catabolism by a combined bacterial association (KRA30) under carbon- and nitrogen-limitations in a retentostat

Theresia Komang Ralebitso-Senior, C. Costa, W F M Roling, Martin Braster, Eric Senior, Henk W van Verseveld

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Aims: Nutrient-limited atrazine catabolism study in continuous cultures with biomass retention to mimic in situ environmental conditions and thus gain insight of the efficacy of biosupplementation/biostimulation to eliminate reduced herbicide bioavailability. Methods and Results: Carbon- and nitrogen-limited retentostat (1 and 5 l) cultivation of a combined atrazine (100 mg l\1)-catabolizing association KRA30 was made. As a nitrogen source, through citrate supplementation, increased herbicide catabolism resulted and was complete in the absence of NH4-N. Co-metabolism of the molecule in the presence of succinate was identified. Population characterization by polymerase chain reaction\denaturing gradient gel electrophoresis (PCR\DGGE) indicated component species numerical dominance shifts in response to changes in nutrient limitation, mineral salts composition and biofilm formation, although the total species complement and catabolic potential were retained. Conclusions: Biomass and catabolic capacity maintenance, through cost-effective biosupplementation/biostimulation, should promote atrazine bioavailability and so ensure successful amelioration. Significance and Impact of the Study: All planning, implementation and monitoring of bioremediation programmes should be underpinned by a combination of molecular and (continuous) culture-based methods.
Original languageEnglish
Pages (from-to)1043-1051
JournalJournal of Applied Microbiology
Volume94
Issue number6
DOIs
Publication statusPublished - 2003

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Atrazine
Nitrogen
Carbon
Herbicides
Biomass
Biological Availability
Food
Denaturing Gradient Gel Electrophoresis
Environmental Biodegradation
Succinic Acid
Biofilms
Citric Acid
Minerals
Salts
Maintenance
Costs and Cost Analysis
Polymerase Chain Reaction
Population

Cite this

Ralebitso-Senior, T. K., Costa, C., Roling, W. F. M., Braster, M., Senior, E., & van Verseveld, H. W. (2003). Atrazine catabolism by a combined bacterial association (KRA30) under carbon- and nitrogen-limitations in a retentostat. Journal of Applied Microbiology, 94(6), 1043-1051. https://doi.org/10.1046/j.1365-2672.2003.01925.x
Ralebitso-Senior, Theresia Komang ; Costa, C. ; Roling, W F M ; Braster, Martin ; Senior, Eric ; van Verseveld, Henk W. / Atrazine catabolism by a combined bacterial association (KRA30) under carbon- and nitrogen-limitations in a retentostat. In: Journal of Applied Microbiology. 2003 ; Vol. 94, No. 6. pp. 1043-1051.
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abstract = "Aims: Nutrient-limited atrazine catabolism study in continuous cultures with biomass retention to mimic in situ environmental conditions and thus gain insight of the efficacy of biosupplementation/biostimulation to eliminate reduced herbicide bioavailability. Methods and Results: Carbon- and nitrogen-limited retentostat (1 and 5 l) cultivation of a combined atrazine (100 mg l\1)-catabolizing association KRA30 was made. As a nitrogen source, through citrate supplementation, increased herbicide catabolism resulted and was complete in the absence of NH4-N. Co-metabolism of the molecule in the presence of succinate was identified. Population characterization by polymerase chain reaction\denaturing gradient gel electrophoresis (PCR\DGGE) indicated component species numerical dominance shifts in response to changes in nutrient limitation, mineral salts composition and biofilm formation, although the total species complement and catabolic potential were retained. Conclusions: Biomass and catabolic capacity maintenance, through cost-effective biosupplementation/biostimulation, should promote atrazine bioavailability and so ensure successful amelioration. Significance and Impact of the Study: All planning, implementation and monitoring of bioremediation programmes should be underpinned by a combination of molecular and (continuous) culture-based methods.",
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Atrazine catabolism by a combined bacterial association (KRA30) under carbon- and nitrogen-limitations in a retentostat. / Ralebitso-Senior, Theresia Komang; Costa, C.; Roling, W F M; Braster, Martin; Senior, Eric; van Verseveld, Henk W.

In: Journal of Applied Microbiology, Vol. 94, No. 6, 2003, p. 1043-1051.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Ralebitso-Senior, Theresia Komang

AU - Costa, C.

AU - Roling, W F M

AU - Braster, Martin

AU - Senior, Eric

AU - van Verseveld, Henk W

PY - 2003

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N2 - Aims: Nutrient-limited atrazine catabolism study in continuous cultures with biomass retention to mimic in situ environmental conditions and thus gain insight of the efficacy of biosupplementation/biostimulation to eliminate reduced herbicide bioavailability. Methods and Results: Carbon- and nitrogen-limited retentostat (1 and 5 l) cultivation of a combined atrazine (100 mg l\1)-catabolizing association KRA30 was made. As a nitrogen source, through citrate supplementation, increased herbicide catabolism resulted and was complete in the absence of NH4-N. Co-metabolism of the molecule in the presence of succinate was identified. Population characterization by polymerase chain reaction\denaturing gradient gel electrophoresis (PCR\DGGE) indicated component species numerical dominance shifts in response to changes in nutrient limitation, mineral salts composition and biofilm formation, although the total species complement and catabolic potential were retained. Conclusions: Biomass and catabolic capacity maintenance, through cost-effective biosupplementation/biostimulation, should promote atrazine bioavailability and so ensure successful amelioration. Significance and Impact of the Study: All planning, implementation and monitoring of bioremediation programmes should be underpinned by a combination of molecular and (continuous) culture-based methods.

AB - Aims: Nutrient-limited atrazine catabolism study in continuous cultures with biomass retention to mimic in situ environmental conditions and thus gain insight of the efficacy of biosupplementation/biostimulation to eliminate reduced herbicide bioavailability. Methods and Results: Carbon- and nitrogen-limited retentostat (1 and 5 l) cultivation of a combined atrazine (100 mg l\1)-catabolizing association KRA30 was made. As a nitrogen source, through citrate supplementation, increased herbicide catabolism resulted and was complete in the absence of NH4-N. Co-metabolism of the molecule in the presence of succinate was identified. Population characterization by polymerase chain reaction\denaturing gradient gel electrophoresis (PCR\DGGE) indicated component species numerical dominance shifts in response to changes in nutrient limitation, mineral salts composition and biofilm formation, although the total species complement and catabolic potential were retained. Conclusions: Biomass and catabolic capacity maintenance, through cost-effective biosupplementation/biostimulation, should promote atrazine bioavailability and so ensure successful amelioration. Significance and Impact of the Study: All planning, implementation and monitoring of bioremediation programmes should be underpinned by a combination of molecular and (continuous) culture-based methods.

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