TY - JOUR
T1 - BBCEAS-HLPC measurements for synthetic antioxidants (TBHQ, BHA, and BHT) in deep-UV region below 300 nm
AU - Bazina, Naser
AU - Ahmed, Tariq Galadanchi
AU - Almdaaf, Mostafa
AU - He, Jibin
AU - Sarker, Mosh
AU - Islam, Meez
N1 - Publisher Copyright:
© 2024 Elsevier Ltd
PY - 2025/2/15
Y1 - 2025/2/15
N2 - This study investigates the integration of Broadband Cavity Enhanced Absorption Spectroscopy (BBCEAS) with High-Performance Liquid Chromatography (HPLC) to detect synthetic antioxidants; BHA, BHT, and TBHQ in the deep-UV region below 300 nm. The research addresses the need for more sensitive and cost-effective detection methods in HPLC systems, particularly for low analyte concentrations, by exploring BBCEAS as a superior alternative to conventional detectors that often fall short in sensitivity. The sensitivity of the HPLC-BBCEAS system is assessed by calculating the minimum detectable change in the absorption coefficient (αmin) and comparing with those obtained using conventional HPLC and single-wavelength HPLC-CRDS methods. The findings demonstrate that the HPLC-BBCEAS system achieves αmin values of 1.8 × 10
−5 cm
−1 for BHA, 2.8 × 10
−5 cm
−1 for BHT, and 1.9 × 10
−5 cm
−1 for TBHQ at 280 nm, with Limits of Detection (LOD) and Quantification (LOQ) up to 30 times lower than conventional HPLC systems.
AB - This study investigates the integration of Broadband Cavity Enhanced Absorption Spectroscopy (BBCEAS) with High-Performance Liquid Chromatography (HPLC) to detect synthetic antioxidants; BHA, BHT, and TBHQ in the deep-UV region below 300 nm. The research addresses the need for more sensitive and cost-effective detection methods in HPLC systems, particularly for low analyte concentrations, by exploring BBCEAS as a superior alternative to conventional detectors that often fall short in sensitivity. The sensitivity of the HPLC-BBCEAS system is assessed by calculating the minimum detectable change in the absorption coefficient (αmin) and comparing with those obtained using conventional HPLC and single-wavelength HPLC-CRDS methods. The findings demonstrate that the HPLC-BBCEAS system achieves αmin values of 1.8 × 10
−5 cm
−1 for BHA, 2.8 × 10
−5 cm
−1 for BHT, and 1.9 × 10
−5 cm
−1 for TBHQ at 280 nm, with Limits of Detection (LOD) and Quantification (LOQ) up to 30 times lower than conventional HPLC systems.
UR - http://www.scopus.com/inward/record.url?scp=85209878140&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2024.142150
DO - 10.1016/j.foodchem.2024.142150
M3 - Article
SN - 0308-8146
VL - 465
JO - Food Chemistry
JF - Food Chemistry
IS - Part 2
M1 - 142150
ER -