Bioremediation of hydrocarbon contaminated gasoline station soil by a bacterial consortium

Pattanathu Rahman, G. Street, R. Lord, G. Kane, I. M. Banat

Research output: Contribution to journalConference articleResearchpeer-review

2 Citations (Scopus)

Abstract

A study was undertaken to find methods for enhancing rates of hydrocarbon biodegradation in gasoline-contaminated soil by ex-situ bioremediation. Garden soil was treated with gasoline-spilled soil from a gasoline station and different combinations of amendments were prepared using mixed bacterial consortium, poultry litter, coir pith and rhamnolipid biosurfactant produced by Pseudomonas aeruginosa. Bacterial growth, hydrocarbon degradation and growth parameters of Phaseolus aureus RoxB (including seed germination, chlorophyll content, shoot and root length) were measured for a period of 90 days. Approximately 78% of the hydrocarbons were effectively degraded within 60 days in soil samples amended with all additives. Maximum germination rate, shoot length, root length and chlorophyll content in Phaseolus aureus were each recorded after 60 days. Further incubation to 90 days did not cause significant improvements. Statistical analysis using Analysis of Variance and Duncan's Multiple Range test revealed that the level of amendments, incubation time and combination of amendments significantly influenced bacterial growth, hydrocarbon degradation, seed germination and chlorophyll content (1% probability level). All tested additives, including rhamnolipid biosurfactant, had significant positive effects on the bioremediation of gasoline-contaminated soils.

Original languageEnglish
Pages (from-to)401-407
Number of pages7
JournalEnvironmental Studies
Volume10
Publication statusPublished - 11 Oct 2004
Event5th International Conference on Environmental Problems in Coastal Regions Incorporating Oil Spill Studies - Alicante, Spain
Duration: 26 Apr 200430 Apr 2004

Fingerprint

gasoline
bioremediation
hydrocarbons
biosurfactants
Vigna radiata var. radiata
chlorophyll
polluted soils
microbial growth
soil
seed germination
coir
shoots
degradation
pith
poultry manure
biodegradation
Pseudomonas aeruginosa
gardens
statistical analysis
soil sampling

Cite this

Rahman, P., Street, G., Lord, R., Kane, G., & Banat, I. M. (2004). Bioremediation of hydrocarbon contaminated gasoline station soil by a bacterial consortium. Environmental Studies, 10, 401-407.
Rahman, Pattanathu ; Street, G. ; Lord, R. ; Kane, G. ; Banat, I. M. / Bioremediation of hydrocarbon contaminated gasoline station soil by a bacterial consortium. In: Environmental Studies. 2004 ; Vol. 10. pp. 401-407.
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abstract = "A study was undertaken to find methods for enhancing rates of hydrocarbon biodegradation in gasoline-contaminated soil by ex-situ bioremediation. Garden soil was treated with gasoline-spilled soil from a gasoline station and different combinations of amendments were prepared using mixed bacterial consortium, poultry litter, coir pith and rhamnolipid biosurfactant produced by Pseudomonas aeruginosa. Bacterial growth, hydrocarbon degradation and growth parameters of Phaseolus aureus RoxB (including seed germination, chlorophyll content, shoot and root length) were measured for a period of 90 days. Approximately 78{\%} of the hydrocarbons were effectively degraded within 60 days in soil samples amended with all additives. Maximum germination rate, shoot length, root length and chlorophyll content in Phaseolus aureus were each recorded after 60 days. Further incubation to 90 days did not cause significant improvements. Statistical analysis using Analysis of Variance and Duncan's Multiple Range test revealed that the level of amendments, incubation time and combination of amendments significantly influenced bacterial growth, hydrocarbon degradation, seed germination and chlorophyll content (1{\%} probability level). All tested additives, including rhamnolipid biosurfactant, had significant positive effects on the bioremediation of gasoline-contaminated soils.",
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Rahman, P, Street, G, Lord, R, Kane, G & Banat, IM 2004, 'Bioremediation of hydrocarbon contaminated gasoline station soil by a bacterial consortium', Environmental Studies, vol. 10, pp. 401-407.

Bioremediation of hydrocarbon contaminated gasoline station soil by a bacterial consortium. / Rahman, Pattanathu; Street, G.; Lord, R.; Kane, G.; Banat, I. M.

In: Environmental Studies, Vol. 10, 11.10.2004, p. 401-407.

Research output: Contribution to journalConference articleResearchpeer-review

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N2 - A study was undertaken to find methods for enhancing rates of hydrocarbon biodegradation in gasoline-contaminated soil by ex-situ bioremediation. Garden soil was treated with gasoline-spilled soil from a gasoline station and different combinations of amendments were prepared using mixed bacterial consortium, poultry litter, coir pith and rhamnolipid biosurfactant produced by Pseudomonas aeruginosa. Bacterial growth, hydrocarbon degradation and growth parameters of Phaseolus aureus RoxB (including seed germination, chlorophyll content, shoot and root length) were measured for a period of 90 days. Approximately 78% of the hydrocarbons were effectively degraded within 60 days in soil samples amended with all additives. Maximum germination rate, shoot length, root length and chlorophyll content in Phaseolus aureus were each recorded after 60 days. Further incubation to 90 days did not cause significant improvements. Statistical analysis using Analysis of Variance and Duncan's Multiple Range test revealed that the level of amendments, incubation time and combination of amendments significantly influenced bacterial growth, hydrocarbon degradation, seed germination and chlorophyll content (1% probability level). All tested additives, including rhamnolipid biosurfactant, had significant positive effects on the bioremediation of gasoline-contaminated soils.

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