Bone mineral change during experimental heating: an X-ray scattering investigation

Jennifer Hiller, Tim J U Thompson, Martin P Evison, Andrew T Chamberlain, Timothy J Wess

    Research output: Contribution to journalArticle

    Abstract

    The effects of heating and burning on bone mineral have previously been studied using techniques such as X-ray diffraction (XRD) with the aim of discerning a characteristic signature of crystal change. This would enable a better understanding of alteration to bone mineral during heating, which would in turn impact on the preparation and use of natural bone hydroxyapatite as a biomaterial resource. In addition, this knowledge could prove invaluable in the investigation of burned human remains from forensic and archaeological contexts in cremation and funerary practice. Here we describe a complementary method, small-angle X-ray scattering (SAXS), to determine more accurately the changes to bone crystallite size and shape during an experimental heating regimen. Samples were subjected to controlled heating at 500°C, 700°C, or 900°C for 15 or 45min. Our results show bone crystallites begin to alter in the first 15min of heating to 500°C or above. They then appear to stabilise to a temperature-specific thickness and shape with prolonged heating. While the samples heated to lower temperatures or for shorter periods produce XRD traces showing little alteration to the apatite, corresponding information obtained from SAXS shows an early, subtle change in crystal parameters.
    Original languageEnglish
    Pages (from-to)5091-5097
    JournalBiomaterials
    Volume24
    Issue number28
    DOIs
    Publication statusPublished - 2003

    Fingerprint Dive into the research topics of 'Bone mineral change during experimental heating: an X-ray scattering investigation'. Together they form a unique fingerprint.

  • Cite this