Ceramides bind VDAC2 to trigger mitochondrial apoptosis

Shashank Dadsena, Svenja Bockelmann, John G. M. Mina, Dina G. Hassan, Sergei Korneev, Guilherme Razzera, Helene Jahn, Patrick Niekamp, Dagmar Müller, Markus Schneider, Fikadu G. Tafesse, Siewert J. Marrink, Manuel N. Melo, Joost C. M. Holthuis

Research output: Contribution to journalArticleResearchpeer-review

3 Downloads (Pure)

Abstract

Ceramides draw wide attention as tumor suppressor lipids that act directly on mitochondria to trigger apoptotic cell death. However, molecular details of the underlying mechanism are largely unknown. Using a photoactivatable ceramide probe, we here identify the voltage-dependent anion channels VDAC1 and VDAC2 as mitochondrial ceramide binding proteins. Coarse-grain molecular dynamics simulations reveal that both channels harbor a ceramide binding site on one side of the barrel wall. This site includes a membrane-buried glutamate that mediates direct contact with the ceramide head group. Substitution or chemical modification of this residue abolishes photolabeling of both channels with the ceramide probe. Unlike VDAC1 removal, loss of VDAC2 or replacing its membrane-facing glutamate with glutamine renders human colon cancer cells largely resistant to ceramide-induced apoptosis. Collectively, our data support a role of VDAC2 as direct effector of ceramide-mediated cell death, providing a molecular framework for how ceramides exert their anti-neoplastic activity.
Original languageEnglish
Article number1832
JournalNature Communications
Volume10
Issue number1
DOIs
Publication statusPublished - 23 Apr 2019

Fingerprint

Ceramides
apoptosis
glutamates
actuators
Apoptosis
death
membranes
glutamine
effectors
suppressors
mitochondria
harbors
probes
lipids
tumors
cancer
Cell death
substitutes
molecular dynamics
anions

Cite this

Dadsena, S., Bockelmann, S., Mina, J. G. M., Hassan, D. G., Korneev, S., Razzera, G., ... Holthuis, J. C. M. (2019). Ceramides bind VDAC2 to trigger mitochondrial apoptosis. Nature Communications, 10(1), [1832]. https://doi.org/10.1038/s41467-019-09654-4
Dadsena, Shashank ; Bockelmann, Svenja ; Mina, John G. M. ; Hassan, Dina G. ; Korneev, Sergei ; Razzera, Guilherme ; Jahn, Helene ; Niekamp, Patrick ; Müller, Dagmar ; Schneider, Markus ; Tafesse, Fikadu G. ; Marrink, Siewert J. ; Melo, Manuel N. ; Holthuis, Joost C. M. / Ceramides bind VDAC2 to trigger mitochondrial apoptosis. In: Nature Communications. 2019 ; Vol. 10, No. 1.
@article{fb3f4106d8034a6fbef61e4ed211e25f,
title = "Ceramides bind VDAC2 to trigger mitochondrial apoptosis",
abstract = "Ceramides draw wide attention as tumor suppressor lipids that act directly on mitochondria to trigger apoptotic cell death. However, molecular details of the underlying mechanism are largely unknown. Using a photoactivatable ceramide probe, we here identify the voltage-dependent anion channels VDAC1 and VDAC2 as mitochondrial ceramide binding proteins. Coarse-grain molecular dynamics simulations reveal that both channels harbor a ceramide binding site on one side of the barrel wall. This site includes a membrane-buried glutamate that mediates direct contact with the ceramide head group. Substitution or chemical modification of this residue abolishes photolabeling of both channels with the ceramide probe. Unlike VDAC1 removal, loss of VDAC2 or replacing its membrane-facing glutamate with glutamine renders human colon cancer cells largely resistant to ceramide-induced apoptosis. Collectively, our data support a role of VDAC2 as direct effector of ceramide-mediated cell death, providing a molecular framework for how ceramides exert their anti-neoplastic activity.",
author = "Shashank Dadsena and Svenja Bockelmann and Mina, {John G. M.} and Hassan, {Dina G.} and Sergei Korneev and Guilherme Razzera and Helene Jahn and Patrick Niekamp and Dagmar M{\"u}ller and Markus Schneider and Tafesse, {Fikadu G.} and Marrink, {Siewert J.} and Melo, {Manuel N.} and Holthuis, {Joost C. M.}",
year = "2019",
month = "4",
day = "23",
doi = "10.1038/s41467-019-09654-4",
language = "English",
volume = "10",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",

}

Dadsena, S, Bockelmann, S, Mina, JGM, Hassan, DG, Korneev, S, Razzera, G, Jahn, H, Niekamp, P, Müller, D, Schneider, M, Tafesse, FG, Marrink, SJ, Melo, MN & Holthuis, JCM 2019, 'Ceramides bind VDAC2 to trigger mitochondrial apoptosis' Nature Communications, vol. 10, no. 1, 1832. https://doi.org/10.1038/s41467-019-09654-4

Ceramides bind VDAC2 to trigger mitochondrial apoptosis. / Dadsena, Shashank; Bockelmann, Svenja; Mina, John G. M.; Hassan, Dina G.; Korneev, Sergei; Razzera, Guilherme; Jahn, Helene; Niekamp, Patrick; Müller, Dagmar; Schneider, Markus; Tafesse, Fikadu G.; Marrink, Siewert J.; Melo, Manuel N.; Holthuis, Joost C. M.

In: Nature Communications, Vol. 10, No. 1, 1832, 23.04.2019.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Ceramides bind VDAC2 to trigger mitochondrial apoptosis

AU - Dadsena, Shashank

AU - Bockelmann, Svenja

AU - Mina, John G. M.

AU - Hassan, Dina G.

AU - Korneev, Sergei

AU - Razzera, Guilherme

AU - Jahn, Helene

AU - Niekamp, Patrick

AU - Müller, Dagmar

AU - Schneider, Markus

AU - Tafesse, Fikadu G.

AU - Marrink, Siewert J.

AU - Melo, Manuel N.

AU - Holthuis, Joost C. M.

PY - 2019/4/23

Y1 - 2019/4/23

N2 - Ceramides draw wide attention as tumor suppressor lipids that act directly on mitochondria to trigger apoptotic cell death. However, molecular details of the underlying mechanism are largely unknown. Using a photoactivatable ceramide probe, we here identify the voltage-dependent anion channels VDAC1 and VDAC2 as mitochondrial ceramide binding proteins. Coarse-grain molecular dynamics simulations reveal that both channels harbor a ceramide binding site on one side of the barrel wall. This site includes a membrane-buried glutamate that mediates direct contact with the ceramide head group. Substitution or chemical modification of this residue abolishes photolabeling of both channels with the ceramide probe. Unlike VDAC1 removal, loss of VDAC2 or replacing its membrane-facing glutamate with glutamine renders human colon cancer cells largely resistant to ceramide-induced apoptosis. Collectively, our data support a role of VDAC2 as direct effector of ceramide-mediated cell death, providing a molecular framework for how ceramides exert their anti-neoplastic activity.

AB - Ceramides draw wide attention as tumor suppressor lipids that act directly on mitochondria to trigger apoptotic cell death. However, molecular details of the underlying mechanism are largely unknown. Using a photoactivatable ceramide probe, we here identify the voltage-dependent anion channels VDAC1 and VDAC2 as mitochondrial ceramide binding proteins. Coarse-grain molecular dynamics simulations reveal that both channels harbor a ceramide binding site on one side of the barrel wall. This site includes a membrane-buried glutamate that mediates direct contact with the ceramide head group. Substitution or chemical modification of this residue abolishes photolabeling of both channels with the ceramide probe. Unlike VDAC1 removal, loss of VDAC2 or replacing its membrane-facing glutamate with glutamine renders human colon cancer cells largely resistant to ceramide-induced apoptosis. Collectively, our data support a role of VDAC2 as direct effector of ceramide-mediated cell death, providing a molecular framework for how ceramides exert their anti-neoplastic activity.

UR - http://www.nature.com/articles/s41467-019-09654-4

UR - http://www.mendeley.com/research/ceramides-bind-vdac2-trigger-mitochondrial-apoptosis

UR - http://www.scopus.com/inward/record.url?scp=85064901827&partnerID=8YFLogxK

U2 - 10.1038/s41467-019-09654-4

DO - 10.1038/s41467-019-09654-4

M3 - Article

VL - 10

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 1832

ER -

Dadsena S, Bockelmann S, Mina JGM, Hassan DG, Korneev S, Razzera G et al. Ceramides bind VDAC2 to trigger mitochondrial apoptosis. Nature Communications. 2019 Apr 23;10(1). 1832. https://doi.org/10.1038/s41467-019-09654-4