Cerebral microvascular permeability and CSF pH in anaesthetized rats

CB Wolf; Mosharraf Sarker; PA Fraser

doi:DOI: 10.1113/expphysiol.1995.sp003903

Cerebral microvascular permeability and CSF pH in anaesthetized rats

CB Wolf, Mosharraf Sarker, PA Fraser

Research output: Contribution to journal › Article › peer-review

Abstract

The idea that the blood-brain barrier (BBB) may be made slightly permeable by an acid load on the brain side was tested in single pial venular capillaries of anaesthetized young rats. The fluorescent dye, Lucifer Yellow (457 Da), was used as a fluid-phase marker to measure permeability in the presence of a free radical scavenger. Tight microvessels were unresponsive to pH changes of 0.3-0.5 units. Vessels that were permeable showed small, significant increases in permeability with decreasing pH (2.19 +/- 0.562 x 10(-6) cm s-1 (pH unit)-1). This effect increased with increasing permeability.

Original language	English
Pages (from-to)	1053-1055
Number of pages	3
Journal	Experimental Physiology
Volume	80
Issue number	6
DOIs	https://doi.org/DOI: 10.1113/expphysiol.1995.sp003903
Publication status	Published - 1 Nov 1995

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title = "Cerebral microvascular permeability and CSF pH in anaesthetized rats",

abstract = "The idea that the blood-brain barrier (BBB) may be made slightly permeable by an acid load on the brain side was tested in single pial venular capillaries of anaesthetized young rats. The fluorescent dye, Lucifer Yellow (457 Da), was used as a fluid-phase marker to measure permeability in the presence of a free radical scavenger. Tight microvessels were unresponsive to pH changes of 0.3-0.5 units. Vessels that were permeable showed small, significant increases in permeability with decreasing pH (2.19 +/- 0.562 x 10(-6) cm s-1 (pH unit)-1). This effect increased with increasing permeability.",

author = "CB Wolf and Mosharraf Sarker and PA Fraser",

year = "1995",

month = nov,

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doi = "DOI: 10.1113/expphysiol.1995.sp003903",

language = "English",

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T1 - Cerebral microvascular permeability and CSF pH in anaesthetized rats

AU - Wolf, CB

AU - Sarker, Mosharraf

AU - Fraser, PA

PY - 1995/11/1

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N2 - The idea that the blood-brain barrier (BBB) may be made slightly permeable by an acid load on the brain side was tested in single pial venular capillaries of anaesthetized young rats. The fluorescent dye, Lucifer Yellow (457 Da), was used as a fluid-phase marker to measure permeability in the presence of a free radical scavenger. Tight microvessels were unresponsive to pH changes of 0.3-0.5 units. Vessels that were permeable showed small, significant increases in permeability with decreasing pH (2.19 +/- 0.562 x 10(-6) cm s-1 (pH unit)-1). This effect increased with increasing permeability.

AB - The idea that the blood-brain barrier (BBB) may be made slightly permeable by an acid load on the brain side was tested in single pial venular capillaries of anaesthetized young rats. The fluorescent dye, Lucifer Yellow (457 Da), was used as a fluid-phase marker to measure permeability in the presence of a free radical scavenger. Tight microvessels were unresponsive to pH changes of 0.3-0.5 units. Vessels that were permeable showed small, significant increases in permeability with decreasing pH (2.19 +/- 0.562 x 10(-6) cm s-1 (pH unit)-1). This effect increased with increasing permeability.

U2 - DOI: 10.1113/expphysiol.1995.sp003903

DO - DOI: 10.1113/expphysiol.1995.sp003903

M3 - Article

SN - 0958-0670

VL - 80

SP - 1053

EP - 1055

JO - Experimental Physiology

JF - Experimental Physiology

IS - 6

ER -

Cerebral microvascular permeability and CSF pH in anaesthetized rats

Abstract

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