Characterization of Bacteria in Ballast Water Using MALDI-TOF Mass Spectrometry

Kaveh Emami, Vahid Askari, Matthias Ullrich, Khwajah Mohinudeen, Arga Chandrashekar Anil, Lidita Khandeparker, J. Grant Burgess, Ehsan Mesbahi, Jonathan H. Badger (Editor)

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Abstract

To evaluate a rapid and cost-effective method for monitoring bacteria in ballast water, several marine bacterial isolates were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Since International Maritime Organization (IMO) regulations are concerned with the unintended transportation of pathogenic bacteria through ballast water, emphasis was placed on detecting species of Vibrio, enterococci and coliforms. Seawater samples collected from the North Sea were incubated in steel ballast tanks and the presence of potentially harmful species of Pseudomonas was also investigated. At the genus-level, the identification of thirty six isolates using MALDI-TOF MS produced similar results to those obtained by 16S rRNA gene sequencing. No pathogenic species were detected either by 16S rRNA gene analysis or by MALDI-TOF MS except for the opportunistically pathogenic bacterium Pseudomonas aeruginosa. In addition, in house software that calculated the correlation coefficient values (CCV) of the mass spectral raw data and their variation was developed and used to allow the rapid and efficient identification of marine bacteria in ballast water for the first time.
Original languageEnglish
Article numbere38515
JournalPLoS ONE
Volume7
Issue number6
DOIs
Publication statusPublished - 7 Jun 2012

Fingerprint

ballast water
matrix-assisted laser desorption-ionization mass spectrometry
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Mass spectrometry
Mass Spectrometry
Bacteria
Ionization
Desorption
Lasers
Water
bacteria
rRNA Genes
Genes
Ballast tanks
Steel tanks
ribosomal RNA
North Sea
Vibrio
Steel
Enterococcus

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Emami, K., Askari, V., Ullrich, M., Mohinudeen, K., Anil, A. C., Khandeparker, L., ... Badger, J. H. (Ed.) (2012). Characterization of Bacteria in Ballast Water Using MALDI-TOF Mass Spectrometry. PLoS ONE, 7(6), [e38515]. https://doi.org/10.1371/journal.pone.0038515
Emami, Kaveh ; Askari, Vahid ; Ullrich, Matthias ; Mohinudeen, Khwajah ; Anil, Arga Chandrashekar ; Khandeparker, Lidita ; Burgess, J. Grant ; Mesbahi, Ehsan ; Badger, Jonathan H. (Editor). / Characterization of Bacteria in Ballast Water Using MALDI-TOF Mass Spectrometry. In: PLoS ONE. 2012 ; Vol. 7, No. 6.
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abstract = "To evaluate a rapid and cost-effective method for monitoring bacteria in ballast water, several marine bacterial isolates were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Since International Maritime Organization (IMO) regulations are concerned with the unintended transportation of pathogenic bacteria through ballast water, emphasis was placed on detecting species of Vibrio, enterococci and coliforms. Seawater samples collected from the North Sea were incubated in steel ballast tanks and the presence of potentially harmful species of Pseudomonas was also investigated. At the genus-level, the identification of thirty six isolates using MALDI-TOF MS produced similar results to those obtained by 16S rRNA gene sequencing. No pathogenic species were detected either by 16S rRNA gene analysis or by MALDI-TOF MS except for the opportunistically pathogenic bacterium Pseudomonas aeruginosa. In addition, in house software that calculated the correlation coefficient values (CCV) of the mass spectral raw data and their variation was developed and used to allow the rapid and efficient identification of marine bacteria in ballast water for the first time.",
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Emami, K, Askari, V, Ullrich, M, Mohinudeen, K, Anil, AC, Khandeparker, L, Burgess, JG, Mesbahi, E & Badger, JH (ed.) 2012, 'Characterization of Bacteria in Ballast Water Using MALDI-TOF Mass Spectrometry', PLoS ONE, vol. 7, no. 6, e38515. https://doi.org/10.1371/journal.pone.0038515

Characterization of Bacteria in Ballast Water Using MALDI-TOF Mass Spectrometry. / Emami, Kaveh; Askari, Vahid; Ullrich, Matthias; Mohinudeen, Khwajah; Anil, Arga Chandrashekar; Khandeparker, Lidita; Burgess, J. Grant; Mesbahi, Ehsan; Badger, Jonathan H. (Editor).

In: PLoS ONE, Vol. 7, No. 6, e38515, 07.06.2012.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Anil, Arga Chandrashekar

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AB - To evaluate a rapid and cost-effective method for monitoring bacteria in ballast water, several marine bacterial isolates were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Since International Maritime Organization (IMO) regulations are concerned with the unintended transportation of pathogenic bacteria through ballast water, emphasis was placed on detecting species of Vibrio, enterococci and coliforms. Seawater samples collected from the North Sea were incubated in steel ballast tanks and the presence of potentially harmful species of Pseudomonas was also investigated. At the genus-level, the identification of thirty six isolates using MALDI-TOF MS produced similar results to those obtained by 16S rRNA gene sequencing. No pathogenic species were detected either by 16S rRNA gene analysis or by MALDI-TOF MS except for the opportunistically pathogenic bacterium Pseudomonas aeruginosa. In addition, in house software that calculated the correlation coefficient values (CCV) of the mass spectral raw data and their variation was developed and used to allow the rapid and efficient identification of marine bacteria in ballast water for the first time.

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Emami K, Askari V, Ullrich M, Mohinudeen K, Anil AC, Khandeparker L et al. Characterization of Bacteria in Ballast Water Using MALDI-TOF Mass Spectrometry. PLoS ONE. 2012 Jun 7;7(6). e38515. https://doi.org/10.1371/journal.pone.0038515