Characterization of hydrocarbon degrading bacteria isolated from Indian crude oil reservoir and their influence on biocorrosion of carbon steel API 5LX

Punniyakotti Parthipan, Punniyakotti Elumalai, Yen Peng Ting, Pattanathu K.S.M. Rahman, Aruliah Rajasekar

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    Abstract

    The role of biosurfactants producing hydrocarbon-degrading bacteria (HDB) on biodegradation and bio-corrosion was evaluated. Biodegradation efficiency (BE) of Streptomyces parvus B7 was found to be 82% when compared to other bacteria. Increased production of biosurfactants directly influences the rate of crude oil BE. Corrosion of carbon steel was found to be more severe in mixed bacterial consortia (1.493 ± 0.015 mm/y). X-ray diffraction confirmed the presence of high intensity of ferric oxide (Fe2O3), iron oxide (Fe3O4), manganese oxide (Mn3O4), and manganese dioxide (MnO2) in corrosion product of mixed bacterial system. Biofilm formation was assist to pit formation on the carbon steel surface and it was evidenced from the atomic force microscopy (AFM) and scanning electron microscopy (SEM) analysis. Corrosion current was increased in the presence of mixed consortia 1.6 ± 0.2 × 10−3 A/cm−2, compared to abiotic control 1.2 ± 0.15 × 10−4 A/cm−2, this values were well supported with charge transfer values and these observations confirmed that mixed bacterial consortia play key role in the corrosion of carbon steel. This is the first report to show degradation of crude oil by Streptomyces parvus B7 and its effects on the corrosion of carbon steel in oil reservoir.

    Original languageEnglish
    Pages (from-to)67-80
    Number of pages14
    JournalInternational Biodeterioration and Biodegradation
    Volume129
    Early online date19 Mar 2018
    DOIs
    Publication statusPublished - 30 Apr 2018

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