Cloning and characterization of the gene cluster required for beauvericin biosynthesis in Fusarium proliferatum

Tao Zhang; Ying Zhuo; Xiaopeng Jia; Jintao Liu; Hong Gao; Fuhang Song; Mei Liu; Lixin Zhang

doi:10.1007/s11427-013-4505-1

Cloning and characterization of the gene cluster required for beauvericin biosynthesis in Fusarium proliferatum

Tao Zhang, Ying Zhuo, Xiaopeng Jia, Jintao Liu, Hong Gao, Fuhang Song, Mei Liu, Lixin Zhang

Research output: Contribution to journal › Article › peer-review

Abstract

Beauvericin, a cyclohexadepsipeptide-possessing natural product with synergistic antifungal, insecticidal, and cytotoxic activities. We isolated and characterized the fpBeas gene cluster, devoted to beauvericin biosynthesis, from the filamentous fungus Fusarium proliferatum LF061. Targeted inactivation of the F. proliferatum genomic copy of fpBeas abolished the production of beauvericin. Comparative sequence analysis of the FpBEAS showed 74% similarity with the BbBEAS that synthesizes the cyclic trimeric ester beauvericin in Beauveria bassiana, which assembles N-methyl-dipeptidol monomer intermediates by the programmed iterative use of the nonribosomal peptide synthetase modules. Differences between the organization of the beauvericin loci in F. proliferaturm and B. bassiana revealed the mechanism for high production of beauvericin in F. proliferatum. Our work provides new insights into beauvericin biosynthesis, and may lead to beauvericin overproduction and creation of new analogs via synthetic biology approaches.

Original language	English
Pages (from-to)	628-37
Number of pages	10
Journal	Science China Life Sciences
Volume	56
Issue number	7
DOIs	https://doi.org/10.1007/s11427-013-4505-1
Publication status	Published - Jul 2013

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title = "Cloning and characterization of the gene cluster required for beauvericin biosynthesis in Fusarium proliferatum",

abstract = "Beauvericin, a cyclohexadepsipeptide-possessing natural product with synergistic antifungal, insecticidal, and cytotoxic activities. We isolated and characterized the fpBeas gene cluster, devoted to beauvericin biosynthesis, from the filamentous fungus Fusarium proliferatum LF061. Targeted inactivation of the F. proliferatum genomic copy of fpBeas abolished the production of beauvericin. Comparative sequence analysis of the FpBEAS showed 74% similarity with the BbBEAS that synthesizes the cyclic trimeric ester beauvericin in Beauveria bassiana, which assembles N-methyl-dipeptidol monomer intermediates by the programmed iterative use of the nonribosomal peptide synthetase modules. Differences between the organization of the beauvericin loci in F. proliferaturm and B. bassiana revealed the mechanism for high production of beauvericin in F. proliferatum. Our work provides new insights into beauvericin biosynthesis, and may lead to beauvericin overproduction and creation of new analogs via synthetic biology approaches.",

author = "Tao Zhang and Ying Zhuo and Xiaopeng Jia and Jintao Liu and Hong Gao and Fuhang Song and Mei Liu and Lixin Zhang",

year = "2013",

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doi = "10.1007/s11427-013-4505-1",

language = "English",

volume = "56",

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T1 - Cloning and characterization of the gene cluster required for beauvericin biosynthesis in Fusarium proliferatum

AU - Zhang, Tao

AU - Zhuo, Ying

AU - Jia, Xiaopeng

AU - Liu, Jintao

AU - Gao, Hong

AU - Song, Fuhang

AU - Liu, Mei

AU - Zhang, Lixin

PY - 2013/7

Y1 - 2013/7

N2 - Beauvericin, a cyclohexadepsipeptide-possessing natural product with synergistic antifungal, insecticidal, and cytotoxic activities. We isolated and characterized the fpBeas gene cluster, devoted to beauvericin biosynthesis, from the filamentous fungus Fusarium proliferatum LF061. Targeted inactivation of the F. proliferatum genomic copy of fpBeas abolished the production of beauvericin. Comparative sequence analysis of the FpBEAS showed 74% similarity with the BbBEAS that synthesizes the cyclic trimeric ester beauvericin in Beauveria bassiana, which assembles N-methyl-dipeptidol monomer intermediates by the programmed iterative use of the nonribosomal peptide synthetase modules. Differences between the organization of the beauvericin loci in F. proliferaturm and B. bassiana revealed the mechanism for high production of beauvericin in F. proliferatum. Our work provides new insights into beauvericin biosynthesis, and may lead to beauvericin overproduction and creation of new analogs via synthetic biology approaches.

AB - Beauvericin, a cyclohexadepsipeptide-possessing natural product with synergistic antifungal, insecticidal, and cytotoxic activities. We isolated and characterized the fpBeas gene cluster, devoted to beauvericin biosynthesis, from the filamentous fungus Fusarium proliferatum LF061. Targeted inactivation of the F. proliferatum genomic copy of fpBeas abolished the production of beauvericin. Comparative sequence analysis of the FpBEAS showed 74% similarity with the BbBEAS that synthesizes the cyclic trimeric ester beauvericin in Beauveria bassiana, which assembles N-methyl-dipeptidol monomer intermediates by the programmed iterative use of the nonribosomal peptide synthetase modules. Differences between the organization of the beauvericin loci in F. proliferaturm and B. bassiana revealed the mechanism for high production of beauvericin in F. proliferatum. Our work provides new insights into beauvericin biosynthesis, and may lead to beauvericin overproduction and creation of new analogs via synthetic biology approaches.

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DO - 10.1007/s11427-013-4505-1

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SN - 1674-7305

VL - 56

SP - 628

EP - 637

JO - Science China Life Sciences

JF - Science China Life Sciences

IS - 7

ER -

Cloning and characterization of the gene cluster required for beauvericin biosynthesis in Fusarium proliferatum

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