Abstract
The intestinal epithelium is formed by a single layer of cells. These cells originate from self-renewal stem cells that give rise to various lineages of cells: Paneth, transit-amplifying, and fully differentiated cells (as enteroendocrine, goblet cells, and enterocytes). Enterocytes, also known as absorptive epithelial cells, are the most abundant cell type in the gut. Enterocytes have the potential to polarize as well as form tight junctions with neighbor cells which altogether serve to ensure both the absorption of “good” substances into the body and the blockage of “bad” substances, among other functions. Culture cell models such as the Caco-2 cell line have been proved to be valuable tools to study the fascinating functions of the intestine. In this chapter we outline some experimental procedures to grow, differentiate, and stain intestinal Caco-2 cells, as well as image them using two modes of confocal laser scanning microscopy.
| Original language | English |
|---|---|
| Title of host publication | Intestinal Differentiated Cells. Methods in Molecular Biology |
| Editors | P Ordóñez-Morán |
| Publisher | Humana Press |
| Pages | 245-259 |
| Number of pages | 15 |
| Volume | 2650 |
| ISBN (Electronic) | 9781071630761 |
| ISBN (Print) | 9781071630754 |
| DOIs | |
| Publication status | Published - 14 Jun 2023 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 2650 |
| ISSN (Print) | 1064-3745 |
| ISSN (Electronic) | 1940-6029 |
Bibliographical note
Funding Information:Caco-2 cells were kindly provided by Dr. Paloma Ordoñez-Morán from the University of Nottingham, UK. This work was supported by grants from the Academy of Medical Science/the Wellcome Trust/the Government Department of Business, Energy and Industrial Strategy/the British Heart Foundation/Diabetes UK AMS Springboard Award [SBF006\1070], and the CIDEGENT Excellence Research Program from the Valencian regional gover-ment CIDEGENT/2021/026 to M.A.J.
Funding Information:
Caco-2 cells were kindly provided by Dr. Paloma Ordoñez-Morán from the University of Nottingham, UK. This work was supported by grants from the Academy of Medical Science/the Wellcome Trust/the Government Department of Business, Energy and Industrial Strategy/the British Heart Foundation/Diabetes UK AMS Springboard Award [SBF006\1070], and the CIDEGENT Excellence Research Program from the Valencian regional gover-ment CIDEGENT/2021/026 to M.A.J
Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.