Detection of regional DNA methylation using DNA-graphene affinity interactions

Md Hakimul Haque, Vinod Gopalan, Sharda Yadav, Md Nazmul Islam, Ehsan Eftekhari, Qin Li, Laura G. Carrascosa, Nam-Trung Nguyen, Alfred K. Lam, Muhammad J. A. Shiddiky

    Research output: Contribution to journalArticlepeer-review


    We report a new method for the detection of regional DNA methylation using base-dependent affinity interaction (i.e., adsorption) of DNA with graphene. Due to the strongest adsorption affinity of guanine bases towards graphene, bisulfite-treated guanine-enriched methylated DNA leads to a larger amount of the adsorbed DNA on the graphene-modified electrodes in comparison to the adenine-enriched unmethylated DNA. The level of the methylation is quantified by monitoring the differential pulse voltammetric current as a function of the adsorbed DNA. The assay is sensitive to distinguish methylated and unmethylated DNA sequences at single CpG resolution by differentiating changes in DNA methylation as low as 5%. Furthermore, this method has been used to detect methylation levels in a collection of DNA samples taken from oesophageal cancer tissues.
    Original languageEnglish
    Pages (from-to)615-621
    JournalBiosensors & Bioelectronics
    Early online date5 Sep 2016
    Publication statusPublished - 15 Jan 2017


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