Development of a marker-free mutagenesis system using CRISPR-Cas9 in the pathogenic mould Aspergillus fumigatus

Keyphrases

• Mutagenesis 100%
• Markerless 100%
• Genome Editing 100%
• Cas9 Protein 100%
• Aspergillus Fumigatus 100%
• Site-directed mutagenesis 66%
• Fumigatus 66%
• Fungal Pathogens 33%
• Disease Progression 33%
• SsODN 33%
• Single-cell PCR 33%
• Gene Editing 33%
• Epitope Tag 33%
• Life-threatening Infections 33%
• Metabolic Burden 33%
• Gene Deletion 33%
• Microhomology 33%
• Repair Template 33%
• Homology Arm 33%
• Saprophytic 33%
• PCR Reaction 33%
• Post-transcriptional Modification 33%
• Protein Tagging 33%
• Selection Marker 33%
• Editing Techniques 33%

Immunology and Microbiology

• Mutagenesis 100%
• Gene Editing 100%
• Mould 100%
• Aspergillus fumigatus 100%
• CRISPR/Cas9 100%
• Site Directed Mutagenesis 50%
• Infectious Agent 25%
• Arm 25%
• Epitope 25%
• DNA Template 25%
• Saprotroph 25%
• Gene Deletion 25%

Agricultural and Biological Sciences

• Site-Directed Mutagenesis 100%