Dissemination of carbapenem resistance and plasmids encoding carbapenemases in Gram-negative bacteria isolated in India

Prasanth Manohar, Sebastian Leptihn, Bruno S. Lopes, Ramesh Nachimuthu

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)
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Abstract

Background: Carbapenem resistance in Gram-negative bacteria is an ongoing public health problem of global dimensions leaving very few treatment options for infected patients. Objectives: To study the dissemination of plasmid-borne carbapenemase genes in Gram-negative bacteria from a diagnostic centre in Tamil Nadu, India. Methods: A total of 151 non-repetitive isolates belonging to 10 genera were collected between January 2015 and December 2016 from a diagnostic centre in Tamil Nadu. The isolates included Escherichia coli (57), Klebsiella pneumoniae (45), Pseudomonas aeruginosa (10), Salmonella Typhi (8), Enterobacter cloacae (8), Acinetobacter baumannii (7), Serratia marcescens (5), Achromobacter xylosoxidans (5), Proteus mirabilis (5), Klebsiella oxytoca (5) and Elizabethkingia meningoseptica (1). Results: Of the 151 isolates, 71% (107) and 68% (103) were found to be resistant to meropenem and imipenem, respectively. The most prevalent β-lactamase gene was blaNDM-1 (22), followed by blaOXA-181 (21), blaGES-1 (11), blaOXA-51 (9), blaGES-9 (8), blaOXA-23 (7) and blaIMP-1 (3). We also observed blaOXA-23 in E. coli (4), and three K. pneumoniae were positive for both, blaOXA-23 and blaOXA-51. Plasmid incompatibility (inc/rep) typing results showed that the resistance genes (11) were present in the isolates carrying plasmid-types IncX, IncA/C, IncFIA-FIB and IncFIIA. The plasmid-borne resistance genes in E. coli and K. pneumoniae were transferred to susceptible E. coli AB1157. Conclusions: This study highlights the prevalence of carbapenem resistance and the acquisition of plasmid-borne carbapenemase genes in Gram-negative bacteria isolated at this centre.

Original languageEnglish
Number of pages9
JournalJAC-Antimicrobial Resistance
Volume3
Issue number1
DOIs
Publication statusPublished - 24 Feb 2021

Bibliographical note

Funding Information:
Financial support was given from the Volkswagenstiftung “Forschung in Museen” to organise the first sorting workshop in Wilhelmshaven July 2016. The second workshop in Spała (Poland) was organised with the support from the internal funds of the University of Lodz and the enthusiasm of all travelling experts. This paper would not be in this special Issue of ZooKeys without the support of the Volkswagenstiftung. Special thanks go to Antje Fischer, who always offered her technical support to solve all issues looking out for vials in the storage rooms and made specimens available to all experts involved at any time. Technical help was also provided by Karen Jeskulke solving all database issues and requests. Leon Kobe did his school practical days during the workshop in Wilhelmshaven and was a great help organizing the workshop as well as Charlotte Brenneken, who provided her student helper assistance in database entries of sorting protocols during this workshop. We thank all workshop participants who could not contribute to paper writing for their participation and sorting effort during the workshops, namely Stas Malavin, Paulina Dębiec and Josh Hatton. Without the enthusiasm of Magda Błażewicz and her team the second workshop in Spała would not have been possible. Species level identification for Oedicerotidae and Amphilochidae was enabled by the Volkswagenstiftung allowing Anne-Nina Lörz, Bente Stransky, and Saskia Brix to invite the specialists for these groups to the DZMB Hamburg for research visits in April 2017 (Lauren Hughes and Ed Hendrycks) and June and August 2017 (Anne Helene S. Tandberg). Travel funds for Tammy Horton were provided by the EU ATLANTOS program. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement no 633211. Alan Myers is thanked for his helpful and constructive review comments on our manuscript.

Funding Information:
Financial support was given from the Volkswagenstiftung “Forschung in Museen” to organise the first sorting workshop in Wilhelmshaven July 2016. The second workshop in Spala (Poland) was organised with the support from the internal funds of the University of Lodz and the enthusiasm of all travelling experts. This paper would not be in this special Issue of ZooKeys without the support of the Volkswagenstiftung. Special thanks go to Antje Fischer, who always offered her technical support to solve all issues looking out for vials in the storage rooms and made specimens available to all experts involved at any time. Technical help was also provided by Karen Jeskulke solving all database issues and requests. Leon Kobe did his school practical days during the workshop in Wilhelmshaven and was a great help organizing the workshop as well as Charlotte Brenneken, who provided her student helper assistance in database entries of sorting protocols during this workshop. We thank all workshop participants who could not contribute to paper writing for their participation and sorting effort during the workshops, namely Stas Malavin, Paulina Debiec and Josh Hatton. Without the enthusiasm of Magda Blazewicz and her team the second workshop in Spala would not have been possible. Species level identification for Oedicerotidae and Amphilochidae was enabled by the Volkswagenstiftung allowing Anne-Nina Lörz, Bente Stransky, and Saskia Brix to invite the specialists for these groups to the DZMB Hamburg for research visits in April2017 (Lauren Hughes and Ed Hendrycks) and June and August 2017 (Anne Helene S. Tandberg). Travel funds for Tammy Horton were provided by the EU ATLANTOS program. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement no 633211. Alan Myers is thanked for his helpful and constructive review comments on our manuscript.

Publisher Copyright:
© 2021 The Author(s). Published by Oxford University Press on behalf of theBritish Society for Antimicrobial Chemotherapy.

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