DNA extraction protocol for animal blood samples using the EZNA blood mini kit.

Louis-Stéphane Le Clercq, Desire Lee Dalton, J. Paul Grobler

Research output: Contribution to journalArticle

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Abstract

The E.Z.N.A. Blood DNA Mini Kit provides an easy and rapid method for the isolation
of genomic DNA for consistent PCR and Southern analysis. Up to 250 μL fresh,
frozen, or anticoagulated whole blood can be readily processed at one time. The
E.Z.N.A. Blood DNA Mini Kit can also be used for the preparation of genomic DNA
from buffy coat, serum, plasma, saliva, buccal swabs, and other body fluids. The
E.Z.N.A. Blood DNA Kit allows for single or multiple simultaneous processing of
multiple samples. There is no need for phenol/chloroform extractions, and timeconsuming steps are eliminated (e.g. precipitation using isopropanol or ethanol).
Purified DNA obtained with the E.Z.N.A. Blood DNA Kit is ready for applications such
as PCR, restriction digestion, and Southern blotting.
Original languageEnglish
JournalProtocols.io
DOIs
Publication statusPublished - 1 Dec 2023

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