Domestication of campylobacter jejuni NCTC 11168

Ben Pascoe; Lisa K. Williams; Jessica K. Calland; Guillaume Meric; Matthew D. Hitchings; Myles Dyer; Joseph Ryder; Sophie Shaw; Bruno S. Lopes; Cosmin Chintoan-Uta; Elaine Allan; Ana Vidal; Catherine Fearnley; Paul Everest; Justin A. Pachebat; Tristan A. Cogan; Mark P. Stevens; Thomas J. Humphrey; Thomas S. Wilkinson; Alison J. Cody; Frances M. Colles; Keith A. Jolley; Martin C.J. Maiden; Norval Strachan; Bruce M. Pearson; Dennis Linton; Brendan W. Wren; Julian Parkhill; David J. Kelly; Arnoud H.M. van Vliet; Ken J. Forbes; Samuel K. Sheppard

doi:10.1099/mgen.0.000279

Domestication of campylobacter jejuni NCTC 11168

Ben Pascoe, Lisa K. Williams, Jessica K. Calland, Guillaume Meric, Matthew D. Hitchings, Myles Dyer, Joseph Ryder, Sophie Shaw, Bruno S. Lopes, Cosmin Chintoan-Uta, Elaine Allan, Ana Vidal, Catherine Fearnley, Paul Everest, Justin A. Pachebat, Tristan A. Cogan, Mark P. Stevens, Thomas J. Humphrey, Thomas S. Wilkinson, Alison J. CodyFrances M. Colles, Keith A. Jolley, Martin C.J. Maiden, Norval Strachan, Bruce M. Pearson, Dennis Linton, Brendan W. Wren, Julian Parkhill, David J. Kelly, Arnoud H.M. van Vliet, Ken J. Forbes, Samuel K. Sheppard

SHLS Life Sciences

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Abstract

Reference and type strains of well-known bacteria have been a cornerstone of microbiology research for decades. The sharing of well-characterized isolates among laboratories has run in parallel with research efforts and enhanced the reproducibility of experiments, leading to a wealth of knowledge about trait variation in different species and the underlying genetics. Campylobacter jejuni strain NCTC 11168, deposited at the National Collection of Type Cultures in 1977, has been adopted widely as a reference strain by researchers worldwide and was the first Campylobacter for which the complete genome was published (in 2000). In this study, we collected 23 C. jejuni NCTC 11168 reference isolates from laboratories across the UK and compared variation in simple laboratory phenotypes with genetic variation in sequenced genomes. Putatively identical isolates, identified previously to have aberrant phenotypes, varied by up to 281 SNPs (in 15 genes) compared to the most recent reference strain. Isolates also display considerable phenotype variation in motility, morphology, growth at 37 °C, invasion of chicken and human cell lines, and susceptibility to ampicillin. This study provides evidence of ongoing evolutionary change among C. jejuni isolates as they are cultured in different laboratories and highlights the need for careful consideration of genetic variation within laboratory reference strains. This article contains data hosted by Microreact.

Original language	English
Article number	000279
Journal	Microbial genomics
Volume	5
Issue number	7
Early online date	3 Jun 2019
DOIs	https://doi.org/10.1099/mgen.0.000279
Publication status	Published - 16 Jul 2019

Bibliographical note

Funding Information:
All high-performance computing was performed on MRC CLIMB, funded by the Medical Research Council (MR/L015080/1). This publication made use of the PubMLST website (http://pubmlst.org/) developed by Keith Jolley and Martin Maiden (Jolley and Maiden, 2010) and sited at the University of Oxford. The development of that website was funded by the Wellcome Trust. We also thank all Campylobacter researchers who have maintained, cultured and disseminated this type strain since its deposition into the NCTC archives in 1977.

Funding Information:
B. P. and S. K. S. are supported by a Medical Research Council grant (MR/L015080/1). L. K. W. is funded by BBSRC (BB/M009610/1). The funders played no part in the study design, article preparation or the decision to publish.

Publisher Copyright:
© 2019 The Authors.

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Pascoe, B., Williams, L. K., Calland, J. K., Meric, G., Hitchings, M. D., Dyer, M., Ryder, J., Shaw, S., Lopes, B. S., Chintoan-Uta, C., Allan, E., Vidal, A., Fearnley, C., Everest, P., Pachebat, J. A., Cogan, T. A., Stevens, M. P., Humphrey, T. J., Wilkinson, T. S., ... Sheppard, S. K. (2019). Domestication of campylobacter jejuni NCTC 11168. Microbial genomics, 5(7), Article 000279. https://doi.org/10.1099/mgen.0.000279

@article{17810dd5b6644473bf5d83152099b70d,

title = "Domestication of campylobacter jejuni NCTC 11168",

abstract = "Reference and type strains of well-known bacteria have been a cornerstone of microbiology research for decades. The sharing of well-characterized isolates among laboratories has run in parallel with research efforts and enhanced the reproducibility of experiments, leading to a wealth of knowledge about trait variation in different species and the underlying genetics. Campylobacter jejuni strain NCTC 11168, deposited at the National Collection of Type Cultures in 1977, has been adopted widely as a reference strain by researchers worldwide and was the first Campylobacter for which the complete genome was published (in 2000). In this study, we collected 23 C. jejuni NCTC 11168 reference isolates from laboratories across the UK and compared variation in simple laboratory phenotypes with genetic variation in sequenced genomes. Putatively identical isolates, identified previously to have aberrant phenotypes, varied by up to 281 SNPs (in 15 genes) compared to the most recent reference strain. Isolates also display considerable phenotype variation in motility, morphology, growth at 37 °C, invasion of chicken and human cell lines, and susceptibility to ampicillin. This study provides evidence of ongoing evolutionary change among C. jejuni isolates as they are cultured in different laboratories and highlights the need for careful consideration of genetic variation within laboratory reference strains. This article contains data hosted by Microreact.",

author = "Ben Pascoe and Williams, {Lisa K.} and Calland, {Jessica K.} and Guillaume Meric and Hitchings, {Matthew D.} and Myles Dyer and Joseph Ryder and Sophie Shaw and Lopes, {Bruno S.} and Cosmin Chintoan-Uta and Elaine Allan and Ana Vidal and Catherine Fearnley and Paul Everest and Pachebat, {Justin A.} and Cogan, {Tristan A.} and Stevens, {Mark P.} and Humphrey, {Thomas J.} and Wilkinson, {Thomas S.} and Cody, {Alison J.} and Colles, {Frances M.} and Jolley, {Keith A.} and Maiden, {Martin C.J.} and Norval Strachan and Pearson, {Bruce M.} and Dennis Linton and Wren, {Brendan W.} and Julian Parkhill and Kelly, {David J.} and {van Vliet}, {Arnoud H.M.} and Forbes, {Ken J.} and Sheppard, {Samuel K.}",

note = "Funding Information: All high-performance computing was performed on MRC CLIMB, funded by the Medical Research Council (MR/L015080/1). This publication made use of the PubMLST website (http://pubmlst.org/) developed by Keith Jolley and Martin Maiden (Jolley and Maiden, 2010) and sited at the University of Oxford. The development of that website was funded by the Wellcome Trust. We also thank all Campylobacter researchers who have maintained, cultured and disseminated this type strain since its deposition into the NCTC archives in 1977. Funding Information: B. P. and S. K. S. are supported by a Medical Research Council grant (MR/L015080/1). L. K. W. is funded by BBSRC (BB/M009610/1). The funders played no part in the study design, article preparation or the decision to publish. Publisher Copyright: {\textcopyright} 2019 The Authors.",

year = "2019",

month = jul,

day = "16",

doi = "10.1099/mgen.0.000279",

language = "English",

volume = "5",

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Pascoe, B, Williams, LK, Calland, JK, Meric, G, Hitchings, MD, Dyer, M, Ryder, J, Shaw, S, Lopes, BS, Chintoan-Uta, C, Allan, E, Vidal, A, Fearnley, C, Everest, P, Pachebat, JA, Cogan, TA, Stevens, MP, Humphrey, TJ, Wilkinson, TS, Cody, AJ, Colles, FM, Jolley, KA, Maiden, MCJ, Strachan, N, Pearson, BM, Linton, D, Wren, BW, Parkhill, J, Kelly, DJ, van Vliet, AHM, Forbes, KJ & Sheppard, SK 2019, 'Domestication of campylobacter jejuni NCTC 11168', Microbial genomics, vol. 5, no. 7, 000279. https://doi.org/10.1099/mgen.0.000279

TY - JOUR

T1 - Domestication of campylobacter jejuni NCTC 11168

AU - Pascoe, Ben

AU - Williams, Lisa K.

AU - Calland, Jessica K.

AU - Meric, Guillaume

AU - Hitchings, Matthew D.

AU - Dyer, Myles

AU - Ryder, Joseph

AU - Shaw, Sophie

AU - Lopes, Bruno S.

AU - Chintoan-Uta, Cosmin

AU - Allan, Elaine

AU - Vidal, Ana

AU - Fearnley, Catherine

AU - Everest, Paul

AU - Pachebat, Justin A.

AU - Cogan, Tristan A.

AU - Stevens, Mark P.

AU - Humphrey, Thomas J.

AU - Wilkinson, Thomas S.

AU - Cody, Alison J.

AU - Colles, Frances M.

AU - Jolley, Keith A.

AU - Maiden, Martin C.J.

AU - Strachan, Norval

AU - Pearson, Bruce M.

AU - Linton, Dennis

AU - Wren, Brendan W.

AU - Parkhill, Julian

AU - Kelly, David J.

AU - van Vliet, Arnoud H.M.

AU - Forbes, Ken J.

AU - Sheppard, Samuel K.

N1 - Funding Information: All high-performance computing was performed on MRC CLIMB, funded by the Medical Research Council (MR/L015080/1). This publication made use of the PubMLST website (http://pubmlst.org/) developed by Keith Jolley and Martin Maiden (Jolley and Maiden, 2010) and sited at the University of Oxford. The development of that website was funded by the Wellcome Trust. We also thank all Campylobacter researchers who have maintained, cultured and disseminated this type strain since its deposition into the NCTC archives in 1977. Funding Information: B. P. and S. K. S. are supported by a Medical Research Council grant (MR/L015080/1). L. K. W. is funded by BBSRC (BB/M009610/1). The funders played no part in the study design, article preparation or the decision to publish. Publisher Copyright: © 2019 The Authors.

PY - 2019/7/16

Y1 - 2019/7/16

N2 - Reference and type strains of well-known bacteria have been a cornerstone of microbiology research for decades. The sharing of well-characterized isolates among laboratories has run in parallel with research efforts and enhanced the reproducibility of experiments, leading to a wealth of knowledge about trait variation in different species and the underlying genetics. Campylobacter jejuni strain NCTC 11168, deposited at the National Collection of Type Cultures in 1977, has been adopted widely as a reference strain by researchers worldwide and was the first Campylobacter for which the complete genome was published (in 2000). In this study, we collected 23 C. jejuni NCTC 11168 reference isolates from laboratories across the UK and compared variation in simple laboratory phenotypes with genetic variation in sequenced genomes. Putatively identical isolates, identified previously to have aberrant phenotypes, varied by up to 281 SNPs (in 15 genes) compared to the most recent reference strain. Isolates also display considerable phenotype variation in motility, morphology, growth at 37 °C, invasion of chicken and human cell lines, and susceptibility to ampicillin. This study provides evidence of ongoing evolutionary change among C. jejuni isolates as they are cultured in different laboratories and highlights the need for careful consideration of genetic variation within laboratory reference strains. This article contains data hosted by Microreact.

AB - Reference and type strains of well-known bacteria have been a cornerstone of microbiology research for decades. The sharing of well-characterized isolates among laboratories has run in parallel with research efforts and enhanced the reproducibility of experiments, leading to a wealth of knowledge about trait variation in different species and the underlying genetics. Campylobacter jejuni strain NCTC 11168, deposited at the National Collection of Type Cultures in 1977, has been adopted widely as a reference strain by researchers worldwide and was the first Campylobacter for which the complete genome was published (in 2000). In this study, we collected 23 C. jejuni NCTC 11168 reference isolates from laboratories across the UK and compared variation in simple laboratory phenotypes with genetic variation in sequenced genomes. Putatively identical isolates, identified previously to have aberrant phenotypes, varied by up to 281 SNPs (in 15 genes) compared to the most recent reference strain. Isolates also display considerable phenotype variation in motility, morphology, growth at 37 °C, invasion of chicken and human cell lines, and susceptibility to ampicillin. This study provides evidence of ongoing evolutionary change among C. jejuni isolates as they are cultured in different laboratories and highlights the need for careful consideration of genetic variation within laboratory reference strains. This article contains data hosted by Microreact.

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DO - 10.1099/mgen.0.000279

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SN - 2057-5858

VL - 5

JO - Microbial genomics

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ER -

Domestication of campylobacter jejuni NCTC 11168

Abstract

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