Doublecortin expression in CD8+ T-cells and microglia at sites of amyloid-β plaques: A potential role in shaping plaque pathology?

Michael S. Unger, Julia Marschallinger, Julia Kaindl, Barbara Klein, Mary Johnson, Ahmad Khundakar, Steffen Roßner, Michael T. Heneka, Sebastien Couillard-Despres, Edward Rockenstein, Eliezer Masliah, Johannes Attems, Ludwig Aigner

    Research output: Contribution to journalArticle

    3 Citations (Scopus)
    157 Downloads (Pure)

    Abstract

    Introduction: One characteristic of Alzheimer's disease is the formation of amyloid-β plaques, which are typically linked to neuroinflammation and surrounded by inflammatory cells such as microglia and infiltrating immune cells. Methods: Here, we describe nonneurogenic doublecortin (DCX) positive cells, DCX being generally used as a marker for young immature neurons, at sites of amyloid-β plaques in various transgenic amyloid mouse models and in human brains with plaque pathology. Results: The plaque-associated DCX+ cells were not of neurogenic identity, instead most of them showed coexpression with markers for microglia (ionized calcium-binding adapter molecule 1) and for phagocytosis (CD68 and TREM2). Another subpopulation of plaque-associated DCX+ cells was negative for ionized calcium-binding adapter molecule 1 but was highly positive for the pan-leukocyte marker CD45. These hematopoietic cells were identified as CD3-and CD8-positive and CD4-negative T-cells. Discussion: Peculiarly, the DCX+/ionized calcium-binding adapter molecule 1+ microglia and DCX+/CD8+ T-cells were closely attached, suggesting that these two cell types are tightly interacting and that this interaction might shape plaque pathology.

    Original languageEnglish
    Pages (from-to)1022-1037
    Number of pages16
    JournalAlzheimer's and Dementia
    Volume14
    Issue number8
    Early online date7 Apr 2018
    DOIs
    Publication statusPublished - 31 Aug 2018

    Fingerprint Dive into the research topics of 'Doublecortin expression in CD8+ T-cells and microglia at sites of amyloid-β plaques: A potential role in shaping plaque pathology?'. Together they form a unique fingerprint.

  • Cite this

    Unger, M. S., Marschallinger, J., Kaindl, J., Klein, B., Johnson, M., Khundakar, A., Roßner, S., Heneka, M. T., Couillard-Despres, S., Rockenstein, E., Masliah, E., Attems, J., & Aigner, L. (2018). Doublecortin expression in CD8+ T-cells and microglia at sites of amyloid-β plaques: A potential role in shaping plaque pathology? Alzheimer's and Dementia, 14(8), 1022-1037. https://doi.org/10.1016/j.jalz.2018.02.017