Early sepsis diagnosis via protein and miRNA biomarkers using a novel point-of-care photonic biosensor

Nuria Fabri-Faja, Olalla Calvo-Lozano, Priyanka Dey, Roland A. Terborg, M.-Carmen Estevez, Alexander Belushkin, Filiz Yesilköy, Luc Duempelmann, Hatice Altug, Valerio Pruneri, Laura M. Lechuga

Research output: Contribution to journalArticlepeer-review


Sepsis is a condition characterized by a severe stage of blood-infection often leading to tissue damage, organ failure and finally death. Fast diagnosis and identification of the sepsis stage (sepsis, severe sepsis or septic shock) is critical for the patient's evolution and could help in defining the most adequate treatment in order to reduce its mortality. The combined detection of several biomarkers in a timely, specific and simultaneous way could ensure a more accurate diagnosis. We have designed a new optical point-of-care (POC) device based on a phase-sensitive interferometric biosensor with a label-free microarray configuration for potential high-throughput evaluation of specific sepsis biomarkers. The sensor chip, which relies on the use of metallic nanostructures, provides versatility in terms of biofunctionalization, allowing the efficient immobilization of different kind of receptors such as antibodies or oligonucleotides. We have focused on two structurally different types of biomarkers: proteins, including C-reactive protein (CRP) and Interleukin 6 (IL6), and miRNAs, using miRNA-16 as an example. Limits of Detection (LoD) of 18 μg mL−1, 88 μg mL−1 and 1 μM (6 μg mL−1) have been respectively obtained for CRP, IL6 and miRNA-16 in individual assays, with high accuracy and reproducibility. The multiplexing capabilities have also been assessed with the simultaneous analysis of both protein biomarkers.
Original languageEnglish
Pages (from-to)232-242
JournalAnalytica Chimica Acta
Early online date20 May 2019
Publication statusPublished - 31 Oct 2019


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