Effects of nitrogen on the distribution and chemical speciation of iron and zinc in pearling fractions of wheat grain

Yan Fang Xue, Tristan Eagling, Jibin He, Chun Qin Zou, Steve P. McGrath, Peter R. Shewry, Fang Jie Zhao

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Increasing nitrogen supply can increase Fe and Zn concentrations in wheat grain, but the underlying mechanisms remain unclear. Size-exclusion chromatography coupled with inductively coupled plasma mass spectrometry was used to determine Fe and Zn speciation in the soluble extracts of grain pearling fractions of two wheat cultivars grown at two N rates (100 and 350 kg of N ha-1). Increasing N supply increased the concentrations of total Fe and Zn and the portions of Fe and Zn unextractable with a Tris-HCl buffer and decreased the concentrations of Tris-HCl-extractable (soluble) Fe and Zn. Within the soluble fraction, Fe and Zn bound to low molecular weight compounds, likely to be Fe-nicotianamine and Fe-deoxymugineic acid or Zn-nicotianamine, were decreased by 5-12% and 4-37%, respectively, by the high N treatment, whereas Fe and Zn bound to soluble high molecular weight or soluble phytate fractions were less affected. The positive effect of N on grain Fe and Zn concentrations was attributed to an increased sink in the grain, probably in the form of water-insoluble proteins.

Original languageEnglish
Pages (from-to)4738-4746
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume62
Issue number20
DOIs
Publication statusPublished - 21 May 2014

Fingerprint

Chemical speciation
chemical speciation
Triticum
Zinc
Nitrogen
Iron
Molecular Weight
zinc
iron
Phytic Acid
Tromethamine
wheat
nitrogen
Gel Chromatography
Mass Spectrometry
Molecular weight
molecular weight
Inductively coupled plasma mass spectrometry
Acids
Size exclusion chromatography

Cite this

Xue, Yan Fang ; Eagling, Tristan ; He, Jibin ; Zou, Chun Qin ; McGrath, Steve P. ; Shewry, Peter R. ; Zhao, Fang Jie. / Effects of nitrogen on the distribution and chemical speciation of iron and zinc in pearling fractions of wheat grain. In: Journal of Agricultural and Food Chemistry. 2014 ; Vol. 62, No. 20. pp. 4738-4746.
@article{f7e4525df7b0425fafd5ad6b2d5230b5,
title = "Effects of nitrogen on the distribution and chemical speciation of iron and zinc in pearling fractions of wheat grain",
abstract = "Increasing nitrogen supply can increase Fe and Zn concentrations in wheat grain, but the underlying mechanisms remain unclear. Size-exclusion chromatography coupled with inductively coupled plasma mass spectrometry was used to determine Fe and Zn speciation in the soluble extracts of grain pearling fractions of two wheat cultivars grown at two N rates (100 and 350 kg of N ha-1). Increasing N supply increased the concentrations of total Fe and Zn and the portions of Fe and Zn unextractable with a Tris-HCl buffer and decreased the concentrations of Tris-HCl-extractable (soluble) Fe and Zn. Within the soluble fraction, Fe and Zn bound to low molecular weight compounds, likely to be Fe-nicotianamine and Fe-deoxymugineic acid or Zn-nicotianamine, were decreased by 5-12{\%} and 4-37{\%}, respectively, by the high N treatment, whereas Fe and Zn bound to soluble high molecular weight or soluble phytate fractions were less affected. The positive effect of N on grain Fe and Zn concentrations was attributed to an increased sink in the grain, probably in the form of water-insoluble proteins.",
author = "Xue, {Yan Fang} and Tristan Eagling and Jibin He and Zou, {Chun Qin} and McGrath, {Steve P.} and Shewry, {Peter R.} and Zhao, {Fang Jie}",
year = "2014",
month = "5",
day = "21",
doi = "10.1021/jf500273x",
language = "English",
volume = "62",
pages = "4738--4746",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "20",

}

Effects of nitrogen on the distribution and chemical speciation of iron and zinc in pearling fractions of wheat grain. / Xue, Yan Fang; Eagling, Tristan; He, Jibin; Zou, Chun Qin; McGrath, Steve P.; Shewry, Peter R.; Zhao, Fang Jie.

In: Journal of Agricultural and Food Chemistry, Vol. 62, No. 20, 21.05.2014, p. 4738-4746.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of nitrogen on the distribution and chemical speciation of iron and zinc in pearling fractions of wheat grain

AU - Xue, Yan Fang

AU - Eagling, Tristan

AU - He, Jibin

AU - Zou, Chun Qin

AU - McGrath, Steve P.

AU - Shewry, Peter R.

AU - Zhao, Fang Jie

PY - 2014/5/21

Y1 - 2014/5/21

N2 - Increasing nitrogen supply can increase Fe and Zn concentrations in wheat grain, but the underlying mechanisms remain unclear. Size-exclusion chromatography coupled with inductively coupled plasma mass spectrometry was used to determine Fe and Zn speciation in the soluble extracts of grain pearling fractions of two wheat cultivars grown at two N rates (100 and 350 kg of N ha-1). Increasing N supply increased the concentrations of total Fe and Zn and the portions of Fe and Zn unextractable with a Tris-HCl buffer and decreased the concentrations of Tris-HCl-extractable (soluble) Fe and Zn. Within the soluble fraction, Fe and Zn bound to low molecular weight compounds, likely to be Fe-nicotianamine and Fe-deoxymugineic acid or Zn-nicotianamine, were decreased by 5-12% and 4-37%, respectively, by the high N treatment, whereas Fe and Zn bound to soluble high molecular weight or soluble phytate fractions were less affected. The positive effect of N on grain Fe and Zn concentrations was attributed to an increased sink in the grain, probably in the form of water-insoluble proteins.

AB - Increasing nitrogen supply can increase Fe and Zn concentrations in wheat grain, but the underlying mechanisms remain unclear. Size-exclusion chromatography coupled with inductively coupled plasma mass spectrometry was used to determine Fe and Zn speciation in the soluble extracts of grain pearling fractions of two wheat cultivars grown at two N rates (100 and 350 kg of N ha-1). Increasing N supply increased the concentrations of total Fe and Zn and the portions of Fe and Zn unextractable with a Tris-HCl buffer and decreased the concentrations of Tris-HCl-extractable (soluble) Fe and Zn. Within the soluble fraction, Fe and Zn bound to low molecular weight compounds, likely to be Fe-nicotianamine and Fe-deoxymugineic acid or Zn-nicotianamine, were decreased by 5-12% and 4-37%, respectively, by the high N treatment, whereas Fe and Zn bound to soluble high molecular weight or soluble phytate fractions were less affected. The positive effect of N on grain Fe and Zn concentrations was attributed to an increased sink in the grain, probably in the form of water-insoluble proteins.

UR - http://www.scopus.com/inward/record.url?scp=84901264468&partnerID=8YFLogxK

U2 - 10.1021/jf500273x

DO - 10.1021/jf500273x

M3 - Article

C2 - 24806959

AN - SCOPUS:84901264468

VL - 62

SP - 4738

EP - 4746

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 20

ER -