Estimating the Reduced Scattering Coefficient of Turbid Media Using Spatially Offset Raman Spectroscopy

Sara Mosca, Priyanka Dey, Marzieh Salimi, Benjamin Gardner, Francesca Palombo, Nick Stone, Pavel Matousek

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Abstract

We propose a new method for estimating the reduced scattering coefficient, μs′, of turbid homogeneous samples using Spatially Offset Raman Spectroscopy (SORS). The concept is based around the variation of Raman signal with SORS spatial offset that is strongly μs′-dependent, as such, permitting the determination of μs′. The evaluation is carried out under the assumptions that absorption is negligible at the laser and Raman wavelengths and μs′ is approximately the same for those two wavelengths. These conditions are often satisfied for samples analyzed in the NIR region of the spectrum where SORS is traditionally deployed. Through a calibration procedure on a PTFE model sample, it was possible to estimate the μs′ coefficient of different turbid samples with an error (RMSEP) below 18%. The knowledge of μs′ in the NIR range is highly valuable for facilitating accurate numerical simulations to optimize illumination and collection geometries in SORS, to derive in-depth information about the properties of SORS measurements or in other photon applications, dependent on photon propagation in turbid media with general impact across fields such as biomedical, pharmaceutical, security, forensic, and cultural sciences.

Original languageEnglish
JournalAnalytical Chemistry
DOIs
Publication statusPublished - 11 Feb 2021
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by the Engineering and Physical Sciences Research Council Grant EP/R020965/1. The authors thank Pranav Lanka and Antonio Pifferi for support in the collection of the spectra of the reduced scattering coefficient shown in the Supporting Information SI2 .

Publisher Copyright:
©

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

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