Exploring Leishmania major Inositol Phosphorylceramide Synthase (LmjIPCS): Insights into the ceramide binding domain

John G. Mina, Jackie A. Mosely, Hayder Z. Ali, Paul W. Denny, Patrick G. Steel

Research output: Contribution to journalArticlepeer-review

Abstract

The synthesis of set of ceramide analogues exploring hydrophobicity in the acyl chains and the degree and nature of hydroxylation is described. These have been assayed against the parasitic protozoan enzymeLmjIPCS. These studies showed that whilst the C-3 hydroxyl group was not essential for turnover it provided enhanced affinity. Reflecting the membrane bound nature of the enzyme a long (C13) hydrocarbon ceramide tail was necessary for both high affinity and turnover. Whilst the N-acyl chain also contributed to affinity, analogues lacking the amide linkage functioned as competitive inhibitors in both enzyme and cell-based assays. A model that accounts for this observation is proposed.
Original languageEnglish
Pages (from-to)1823-1830
Number of pages8
JournalOrganic and Biomolecular Chemistry
Volume9
Issue number6
DOIs
Publication statusPublished - 2011

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