Expression profile of human tissue kallikrein 15 provides preliminary insights into its roles in the prostate and testis

Panagiota S. Filippou, Annie H. Ren, Antoninus Soosaipillai, Michail Dimitrios Papaioannou, Dimitrios Korbakis, Roaa Safar, Eleftherios P. Diamandis, James R. Conner

Research output: Contribution to journalArticle

Abstract

Background: Human tissue kallikrein 15 (KLK15) is the latest member of the kallikrein-related peptidase family. Little is known about the pathophysiological roles of KLK15. Previous studies implied a role of KLK15 in prostate cancer. Methods: In the present study, we examined KLK15 protein expression using a new immunoassay (ELISA) and immunohistochemistry (IHC). Results: Highest KLK15 levels were detected in the testis and seminal fluid, whereas lower levels were observed in prostate and other tissues. Immunohistochemical analysis of testis suggests that KLK15 is strongly expressed in mature spermatids, but not in immature germ cells. KLK15 displayed predominantly nuclear localization in the basal cell layer of the prostatic epithelium. We also measured KLK15 in supernatants of various cell lines. Highest KLK15 levels were primarily detected in prostate cancer cell lines and KLK15 expression was hormone-independent, in contrast to KLK3. Conclusions: Collectively, our data provide insights into the localization and possible role of KLK15 in human physiology.

Original languageEnglish
Pages (from-to)78-85
Number of pages8
JournalClinical Biochemistry
Volume59
Early online date26 Jun 2018
DOIs
Publication statusPublished - 1 Sep 2018

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Tissue Kallikreins
Kallikreins
Testis
Prostate
Cells
Prostatic Neoplasms
human KLK15 protein
Cell Line
Spermatids
Physiology
Immunoassay
Germ Cells
Peptide Hydrolases
Epithelium
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Hormones

Bibliographical note

Copyright © 2018 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

Cite this

Filippou, Panagiota S. ; Ren, Annie H. ; Soosaipillai, Antoninus ; Papaioannou, Michail Dimitrios ; Korbakis, Dimitrios ; Safar, Roaa ; Diamandis, Eleftherios P. ; Conner, James R. / Expression profile of human tissue kallikrein 15 provides preliminary insights into its roles in the prostate and testis. In: Clinical Biochemistry. 2018 ; Vol. 59. pp. 78-85.
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Expression profile of human tissue kallikrein 15 provides preliminary insights into its roles in the prostate and testis. / Filippou, Panagiota S.; Ren, Annie H.; Soosaipillai, Antoninus; Papaioannou, Michail Dimitrios; Korbakis, Dimitrios; Safar, Roaa; Diamandis, Eleftherios P.; Conner, James R.

In: Clinical Biochemistry, Vol. 59, 01.09.2018, p. 78-85.

Research output: Contribution to journalArticle

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AU - Ren, Annie H.

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AU - Papaioannou, Michail Dimitrios

AU - Korbakis, Dimitrios

AU - Safar, Roaa

AU - Diamandis, Eleftherios P.

AU - Conner, James R.

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