TY - JOUR
T1 - Expression profile of human tissue kallikrein 15 provides preliminary insights into its roles in the prostate and testis
AU - Filippou, Panagiota S.
AU - Ren, Annie H.
AU - Soosaipillai, Antoninus
AU - Papaioannou, Michail Dimitrios
AU - Korbakis, Dimitrios
AU - Safar, Roaa
AU - Diamandis, Eleftherios P.
AU - Conner, James R.
N1 - Copyright © 2018 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
PY - 2018/9/1
Y1 - 2018/9/1
N2 - Background: Human tissue kallikrein 15 (KLK15) is the latest member of the kallikrein-related peptidase family. Little is known about the pathophysiological roles of KLK15. Previous studies implied a role of KLK15 in prostate cancer. Methods: In the present study, we examined KLK15 protein expression using a new immunoassay (ELISA) and immunohistochemistry (IHC). Results: Highest KLK15 levels were detected in the testis and seminal fluid, whereas lower levels were observed in prostate and other tissues. Immunohistochemical analysis of testis suggests that KLK15 is strongly expressed in mature spermatids, but not in immature germ cells. KLK15 displayed predominantly nuclear localization in the basal cell layer of the prostatic epithelium. We also measured KLK15 in supernatants of various cell lines. Highest KLK15 levels were primarily detected in prostate cancer cell lines and KLK15 expression was hormone-independent, in contrast to KLK3. Conclusions: Collectively, our data provide insights into the localization and possible role of KLK15 in human physiology.
AB - Background: Human tissue kallikrein 15 (KLK15) is the latest member of the kallikrein-related peptidase family. Little is known about the pathophysiological roles of KLK15. Previous studies implied a role of KLK15 in prostate cancer. Methods: In the present study, we examined KLK15 protein expression using a new immunoassay (ELISA) and immunohistochemistry (IHC). Results: Highest KLK15 levels were detected in the testis and seminal fluid, whereas lower levels were observed in prostate and other tissues. Immunohistochemical analysis of testis suggests that KLK15 is strongly expressed in mature spermatids, but not in immature germ cells. KLK15 displayed predominantly nuclear localization in the basal cell layer of the prostatic epithelium. We also measured KLK15 in supernatants of various cell lines. Highest KLK15 levels were primarily detected in prostate cancer cell lines and KLK15 expression was hormone-independent, in contrast to KLK3. Conclusions: Collectively, our data provide insights into the localization and possible role of KLK15 in human physiology.
UR - http://www.scopus.com/inward/record.url?scp=85049919291&partnerID=8YFLogxK
U2 - 10.1016/j.clinbiochem.2018.06.017
DO - 10.1016/j.clinbiochem.2018.06.017
M3 - Article
C2 - 29958881
AN - SCOPUS:85049919291
SN - 0009-9120
VL - 59
SP - 78
EP - 85
JO - Clinical Biochemistry
JF - Clinical Biochemistry
ER -