Fluorescence quenched quinone methide based activity probes - A cautionary tale

Jonathan D. Sellars; Marie Landrum; Aileen Congreve; David P. Dixon; Jackie A. Mosely; Andrew Beeby; Robert Edwards; Patrick G. Steel

doi:10.1039/b920443a

Fluorescence quenched quinone methide based activity probes - A cautionary tale

Jonathan D. Sellars, Marie Landrum, Aileen Congreve, David P. Dixon, Jackie A. Mosely, Andrew Beeby, Robert Edwards, Patrick G. Steel

Centre for Biodiscovery

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

A carbamate linked quenching group coupled with a pro-quinone methide reactive core provides an effective tool for studying enzyme function without problems associated with background fluorescence from unreacted probe. However, the relatively slow fragmentation of the carbamate linkage in such a strategy may cause problems of loss of signal or a decoupling of enzyme activity and labelling.

Original language	English
Pages (from-to)	1610-1618
Number of pages	9
Journal	Organic and Biomolecular Chemistry
Volume	8
Issue number	7
DOIs	https://doi.org/10.1039/b920443a
Publication status	Published - 2010

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Copyright 2010 Elsevier B.V., All rights reserved.

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AU - Beeby, Andrew

AU - Edwards, Robert

AU - Steel, Patrick G.

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AB - A carbamate linked quenching group coupled with a pro-quinone methide reactive core provides an effective tool for studying enzyme function without problems associated with background fluorescence from unreacted probe. However, the relatively slow fragmentation of the carbamate linkage in such a strategy may cause problems of loss of signal or a decoupling of enzyme activity and labelling.

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Fluorescence quenched quinone methide based activity probes - A cautionary tale

Abstract

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