Fluorescence quenched quinone methide based activity probes - A cautionary tale

Jonathan D. Sellars, Marie Landrum, Aileen Congreve, David P. Dixon, Jackie A. Mosely, Andrew Beeby, Robert Edwards, Patrick G. Steel

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


A carbamate linked quenching group coupled with a pro-quinone methide reactive core provides an effective tool for studying enzyme function without problems associated with background fluorescence from unreacted probe. However, the relatively slow fragmentation of the carbamate linkage in such a strategy may cause problems of loss of signal or a decoupling of enzyme activity and labelling.

Original languageEnglish
Pages (from-to)1610-1618
Number of pages9
JournalOrganic and Biomolecular Chemistry
Issue number7
Publication statusPublished - 2010

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Copyright 2010 Elsevier B.V., All rights reserved.


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