Fluoroimmunoassay of influenza virus using sulfur-doped graphitic carbon nitride quantum dots coupled with Ag2S nanocrystals

Ojodomo J. Achadu; De Xing Lioe; Keiichiro Kagawa; Shoji Kawahito; Enoch Y. Park

doi:10.1007/s00604-020-04433-1

Fluoroimmunoassay of influenza virus using sulfur-doped graphitic carbon nitride quantum dots coupled with Ag₂S nanocrystals

Ojodomo J. Achadu, De Xing Lioe, Keiichiro Kagawa, Shoji Kawahito, Enoch Y. Park

SHLS Life Sciences

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Novel sulfur-doped graphitic carbon nitride quantum dots (S-gCNQDs) are synthesized using a single-source precursor in a one-step solvothermal process. The S-gCNQDs with a size of ~ 5-nm displayed a strong green intrinsic fluorescence at 512 nm when excited at 400 nm, with a quantum yield of ~ 33% in aqueous solution. The prepared S-gCNQDs and Ag₂S nanocrystals were applied as innovative functional materials to fabricate a biosensor for virus detection based on the conjugation of specific anti-human influenza A monoclonal antibody to the S-gCNQDs and Ag₂S NCs, respectively. In the presence of the influenza A virus, an interaction between the S-gCNQDs/Ag₂S-labeled antibody resulted in the formation of a nanosandwich structure, which is accompanied by the fluorescence enhancement of the S-gCNQDs. The change in fluorescence intensity linearly correlats with the concentration of the influenza A virus (H1N1) in the 10 fg/mL to 1.0 ng/mL range, with a limit of detection of 5.5 fg/mL. The assay was applied to the assay of clinically isolated influenza A virus (H3N2/Yokohama) mixed with human serum. The obtained limit of detection was 100 PFU/mL within the detection range of 10²– 5 × 10⁴ PFU/mL for the H3N2 virus. [Figure not available: see fulltext.].

Original language	English
Article number	466
Journal	Microchimica Acta
Volume	187
Issue number	8
DOIs	https://doi.org/10.1007/s00604-020-04433-1
Publication status	Published - 21 Jul 2020

Bibliographical note

Funding Information:
The authors sincerely thank Professor K. Morita of the Institute of Tropical Medicine, Nagasaki University, for providing the Zika virus. Dr. C. Kawakami of Yokohama City Institute of Health, Japan, is gratefully acknowledged for providing the clinically isolated influenza virus A/Yokohama/110/2009 (H3N2). We also wish to thank Dr. T.C. Li of the National Institute for Infectious Disease of Japan for providing HEV-LPs.

Funding Information:
O.J.A and S. K. gratefully acknowledge the Japan Society for the Promotion of Science (JSPS) for a Postdoctoral Fellowship for Research in Japan (Standard) (Grant No. 19F19348) and the Grant-in-Aid for Scientific Research (S) (Grant No. 18H05240), respectively. Acknowledgments

Publisher Copyright:
© 2020, Springer-Verlag GmbH Austria, part of Springer Nature.

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title = "Fluoroimmunoassay of influenza virus using sulfur-doped graphitic carbon nitride quantum dots coupled with Ag2S nanocrystals",

abstract = "Novel sulfur-doped graphitic carbon nitride quantum dots (S-gCNQDs) are synthesized using a single-source precursor in a one-step solvothermal process. The S-gCNQDs with a size of ~ 5-nm displayed a strong green intrinsic fluorescence at 512 nm when excited at 400 nm, with a quantum yield of ~ 33% in aqueous solution. The prepared S-gCNQDs and Ag2S nanocrystals were applied as innovative functional materials to fabricate a biosensor for virus detection based on the conjugation of specific anti-human influenza A monoclonal antibody to the S-gCNQDs and Ag2S NCs, respectively. In the presence of the influenza A virus, an interaction between the S-gCNQDs/Ag2S-labeled antibody resulted in the formation of a nanosandwich structure, which is accompanied by the fluorescence enhancement of the S-gCNQDs. The change in fluorescence intensity linearly correlats with the concentration of the influenza A virus (H1N1) in the 10 fg/mL to 1.0 ng/mL range, with a limit of detection of 5.5 fg/mL. The assay was applied to the assay of clinically isolated influenza A virus (H3N2/Yokohama) mixed with human serum. The obtained limit of detection was 100 PFU/mL within the detection range of 102– 5 × 104 PFU/mL for the H3N2 virus. [Figure not available: see fulltext.].",

author = "Achadu, {Ojodomo J.} and Lioe, {De Xing} and Keiichiro Kagawa and Shoji Kawahito and Park, {Enoch Y.}",

note = "Funding Information: The authors sincerely thank Professor K. Morita of the Institute of Tropical Medicine, Nagasaki University, for providing the Zika virus. Dr. C. Kawakami of Yokohama City Institute of Health, Japan, is gratefully acknowledged for providing the clinically isolated influenza virus A/Yokohama/110/2009 (H3N2). We also wish to thank Dr. T.C. Li of the National Institute for Infectious Disease of Japan for providing HEV-LPs. Funding Information: O.J.A and S. K. gratefully acknowledge the Japan Society for the Promotion of Science (JSPS) for a Postdoctoral Fellowship for Research in Japan (Standard) (Grant No. 19F19348) and the Grant-in-Aid for Scientific Research (S) (Grant No. 18H05240), respectively. Acknowledgments Publisher Copyright: {\textcopyright} 2020, Springer-Verlag GmbH Austria, part of Springer Nature.",

year = "2020",

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doi = "10.1007/s00604-020-04433-1",

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T1 - Fluoroimmunoassay of influenza virus using sulfur-doped graphitic carbon nitride quantum dots coupled with Ag2S nanocrystals

AU - Achadu, Ojodomo J.

AU - Lioe, De Xing

AU - Kagawa, Keiichiro

AU - Kawahito, Shoji

AU - Park, Enoch Y.

N1 - Funding Information: The authors sincerely thank Professor K. Morita of the Institute of Tropical Medicine, Nagasaki University, for providing the Zika virus. Dr. C. Kawakami of Yokohama City Institute of Health, Japan, is gratefully acknowledged for providing the clinically isolated influenza virus A/Yokohama/110/2009 (H3N2). We also wish to thank Dr. T.C. Li of the National Institute for Infectious Disease of Japan for providing HEV-LPs. Funding Information: O.J.A and S. K. gratefully acknowledge the Japan Society for the Promotion of Science (JSPS) for a Postdoctoral Fellowship for Research in Japan (Standard) (Grant No. 19F19348) and the Grant-in-Aid for Scientific Research (S) (Grant No. 18H05240), respectively. Acknowledgments Publisher Copyright: © 2020, Springer-Verlag GmbH Austria, part of Springer Nature.

PY - 2020/7/21

Y1 - 2020/7/21

N2 - Novel sulfur-doped graphitic carbon nitride quantum dots (S-gCNQDs) are synthesized using a single-source precursor in a one-step solvothermal process. The S-gCNQDs with a size of ~ 5-nm displayed a strong green intrinsic fluorescence at 512 nm when excited at 400 nm, with a quantum yield of ~ 33% in aqueous solution. The prepared S-gCNQDs and Ag2S nanocrystals were applied as innovative functional materials to fabricate a biosensor for virus detection based on the conjugation of specific anti-human influenza A monoclonal antibody to the S-gCNQDs and Ag2S NCs, respectively. In the presence of the influenza A virus, an interaction between the S-gCNQDs/Ag2S-labeled antibody resulted in the formation of a nanosandwich structure, which is accompanied by the fluorescence enhancement of the S-gCNQDs. The change in fluorescence intensity linearly correlats with the concentration of the influenza A virus (H1N1) in the 10 fg/mL to 1.0 ng/mL range, with a limit of detection of 5.5 fg/mL. The assay was applied to the assay of clinically isolated influenza A virus (H3N2/Yokohama) mixed with human serum. The obtained limit of detection was 100 PFU/mL within the detection range of 102– 5 × 104 PFU/mL for the H3N2 virus. [Figure not available: see fulltext.].

AB - Novel sulfur-doped graphitic carbon nitride quantum dots (S-gCNQDs) are synthesized using a single-source precursor in a one-step solvothermal process. The S-gCNQDs with a size of ~ 5-nm displayed a strong green intrinsic fluorescence at 512 nm when excited at 400 nm, with a quantum yield of ~ 33% in aqueous solution. The prepared S-gCNQDs and Ag2S nanocrystals were applied as innovative functional materials to fabricate a biosensor for virus detection based on the conjugation of specific anti-human influenza A monoclonal antibody to the S-gCNQDs and Ag2S NCs, respectively. In the presence of the influenza A virus, an interaction between the S-gCNQDs/Ag2S-labeled antibody resulted in the formation of a nanosandwich structure, which is accompanied by the fluorescence enhancement of the S-gCNQDs. The change in fluorescence intensity linearly correlats with the concentration of the influenza A virus (H1N1) in the 10 fg/mL to 1.0 ng/mL range, with a limit of detection of 5.5 fg/mL. The assay was applied to the assay of clinically isolated influenza A virus (H3N2/Yokohama) mixed with human serum. The obtained limit of detection was 100 PFU/mL within the detection range of 102– 5 × 104 PFU/mL for the H3N2 virus. [Figure not available: see fulltext.].

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Fluoroimmunoassay of influenza virus using sulfur-doped graphitic carbon nitride quantum dots coupled with Ag₂S nanocrystals

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