Gold-Loaded Nanoporous Ferric Oxide Nanocubes with Peroxidase-Mimicking Activity for Electrocatalytic and Colorimetric Detection of Autoantibody

Mostafa Kamal Masud, Sharda Yadav, Md Nazmul Islam, Nam-Trung Nguyen, Carlos Salomon, Hatem R Almari, Zeid A Alothman, Yusuke Yamauchi, Md Shahriar A Hossain, Muhammad J A Shiddiky

    Research output: Contribution to journalArticle

    Abstract

    The enzyme-mimicking activity of iron oxide based nanostructures has provided a significant advantage in developing advanced molecular sensors for biomedical and environmental applications. Herein, we introduce the horseradish peroxidase (HRP)-like activity of gold-loaded nanoporous ferric oxide nanocubes (Au–NPFe2O3NC) for the development of a molecular sensor with enhanced electrocatalytic and colorimetric (naked eye) detection of autoantibodies. The results showed that Au–NPFe2O3NC exhibits enhanced peroxidase-like activity toward the catalytic oxidation of 3,3′,5,5′-tertamethylbenzidine (TMB) in the presence of H2O2 at room temperature (25 °C) and follows the typical Michaelis–Menten kinetics. The autoantibody sensor based on this intrinsic property of Au–NPFe2O3NC resulted in excellent detection sensitivity [limit of detection (LOD) = 0.08 U/mL] and reproducibility [percent relative standard deviation (% RSD) = <5% for n = 3] for analyzing p53-specific autoantibodies using electrochemical and colorimetric (naked eye) readouts. The clinical applicability of the sensor has been tested in detecting p53-specific autoantibody in plasma obtained from patients with epithelial ovarian cancer high-grade serous subtype (EOCHGS, number of samples = 2) and controls (benign, number of samples = 2). As Au–NPFe2O3NC possess high peroxidase-like activity for the oxidation of TMB in the presence of H2O2 [TMB is a common chromogenic substrate for HRP in enzyme-linked immunosorbent assays (ELISAs)], we envisage that our assay could find a wide range of application in developing ELISA-based sensing approaches in the fields of medicine (i.e., detection of other biomarkers the same as p53 autoantibody), biotechnology, and environmental sciences.
    Original languageEnglish
    Pages (from-to)11005-11013
    Number of pages9
    JournalAnalytical Chemistry
    Volume89
    Issue number20
    DOIs
    Publication statusPublished - 11 Sep 2017

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    Masud, M. K., Yadav, S., Islam, M. N., Nguyen, N-T., Salomon, C., Almari, H. R., Alothman, Z. A., Yamauchi, Y., Hossain, M. S. A., & Shiddiky, M. J. A. (2017). Gold-Loaded Nanoporous Ferric Oxide Nanocubes with Peroxidase-Mimicking Activity for Electrocatalytic and Colorimetric Detection of Autoantibody. Analytical Chemistry, 89(20), 11005-11013. https://doi.org/10.1021/acs.analchem.7b02880