Identification of avermectin-high-producing strains by high-throughput screening methods

Hong Gao, Mei Liu, Xianlong Zhou, Jintao Liu, Ying Zhuo, Zhongxuan Gou, Bing Xu, Wenquan Zhang, Xiangyang Liu, Aiqun Luo, Chuansen Zheng, Xiaoping Chen, Lixin Zhang

    Research output: Contribution to journalArticlepeer-review


    Avermectins produced by Streptomyces avermitilis are potent against a broad spectrum of nematode and arthropod parasites with low-level side effects on the host organisms. This study was designed to investigate a high-throughput screening strategy for the efficient identification of avermectin high-yield strains. The production protocol was miniaturized in 96 deep-well microplates. UV absorbance at 245 nm was used to monitor avermectin production. A good correlation between fermentation results in both 96 deep-well microplates and conventional Erlenmeyer flasks was observed. With this protocol, the production of avermectins was determined in less than 10 min for a full plate without compromising accuracy. The high-yield strain selected through this protocol was also tested in 360 m(3) batch fermentation with 1.6-fold improved outcome. Thus, the development of this protocol is expected to accelerate the selection of superior avermectin-producing strains.

    Original languageEnglish
    Pages (from-to)1219-25
    Number of pages7
    JournalApplied Microbiology and Biotechnology
    Issue number4
    Publication statusPublished - Jan 2010


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