Impedimetric array in polymer microfluidic cartridge for low cost point-of-care diagnostics

Andrew Lakey, Zulfiqur Ali, Simon Scott, Syrine Chebil, Hafsa Korri-Youssoufi, Santha Hunor, Anna Ohlander, Mathias Kuphal, Josep Samitier Marti

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Deep Vein Thrombosis and pulmonary embolism (DVT/PE) is one of the most common causes of unexpected death for hospital in-patients. D-dimer is used as a biomarker within blood for the diagnosis of DVT/PE. We report a low-cost microfluidic device with a conveniently biofunctionalised interdigitated electrode (IDE) array and a portable impedimetric reader as a point-of-care (POC) device for the detection of D-dimer to aid diagnosis of DVT/PE. The IDE array elements, fabricated on a polyethylenenaphtalate (PEN) substrate, are biofunctionalised in situ after assembly of the microfluidic device by electropolymerisation of a copolymer of polypyrrole to which is immobilised a histidine tag anti-D-Dimer antibody. The most consistent copolymer films were produced using chronopotentiometry with an applied current of 5A for a period of 50 seconds using a two-electrode system. The quality of the biofunctionalisation was monitored using optical microscopy, chronopotentiometry curves and impedimetric analysis. Measurement of clinical plasma sample with a D-dimer at concentration of 437 ng/mL with 15 biofunctionalised IDE array electrodes gave a ratiometric percentage of sample reading against the blank with an average value of 124±15 at 95% confidence. We have demonstrated the concept of a low cost disposable microfluidic device with a receptor functionalised on the IDE array for impedimetric detection towards POC diagnostics. Changing the receptor on the IDE array would allow this approach to be used for the direct detection of a wide range of analytes in a low cost manner.
LanguageEnglish
Pages147-154
Number of pages8
JournalBiosensors and Bioelectronics
Volume129
Early online date11 Jan 2019
DOIs
Publication statusE-pub ahead of print - 11 Jan 2019

Fingerprint

Point-of-Care Systems
Microfluidics
Lab-On-A-Chip Devices
Polymers
Electrodes
Costs and Cost Analysis
Dimers
Costs
Pulmonary Embolism
Venous Thrombosis
Copolymers
Electropolymerization
Polypyrroles
Biomarkers
Histidine
Antibodies
Optical microscopy
Reading
Cause of Death
Microscopy

Cite this

Lakey, Andrew ; Ali, Zulfiqur ; Scott, Simon ; Chebil, Syrine ; Korri-Youssoufi, Hafsa ; Hunor, Santha ; Ohlander, Anna ; Kuphal, Mathias ; Samitier Marti, Josep. / Impedimetric array in polymer microfluidic cartridge for low cost point-of-care diagnostics. In: Biosensors and Bioelectronics. 2019 ; Vol. 129. pp. 147-154.
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abstract = "Deep Vein Thrombosis and pulmonary embolism (DVT/PE) is one of the most common causes of unexpected death for hospital in-patients. D-dimer is used as a biomarker within blood for the diagnosis of DVT/PE. We report a low-cost microfluidic device with a conveniently biofunctionalised interdigitated electrode (IDE) array and a portable impedimetric reader as a point-of-care (POC) device for the detection of D-dimer to aid diagnosis of DVT/PE. The IDE array elements, fabricated on a polyethylenenaphtalate (PEN) substrate, are biofunctionalised in situ after assembly of the microfluidic device by electropolymerisation of a copolymer of polypyrrole to which is immobilised a histidine tag anti-D-Dimer antibody. The most consistent copolymer films were produced using chronopotentiometry with an applied current of 5A for a period of 50 seconds using a two-electrode system. The quality of the biofunctionalisation was monitored using optical microscopy, chronopotentiometry curves and impedimetric analysis. Measurement of clinical plasma sample with a D-dimer at concentration of 437 ng/mL with 15 biofunctionalised IDE array electrodes gave a ratiometric percentage of sample reading against the blank with an average value of 124±15 at 95{\%} confidence. We have demonstrated the concept of a low cost disposable microfluidic device with a receptor functionalised on the IDE array for impedimetric detection towards POC diagnostics. Changing the receptor on the IDE array would allow this approach to be used for the direct detection of a wide range of analytes in a low cost manner.",
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Lakey, A, Ali, Z, Scott, S, Chebil, S, Korri-Youssoufi, H, Hunor, S, Ohlander, A, Kuphal, M & Samitier Marti, J 2019, 'Impedimetric array in polymer microfluidic cartridge for low cost point-of-care diagnostics', Biosensors and Bioelectronics, vol. 129, pp. 147-154. https://doi.org/10.1016/j.bios.2018.12.054

Impedimetric array in polymer microfluidic cartridge for low cost point-of-care diagnostics. / Lakey, Andrew; Ali, Zulfiqur; Scott, Simon; Chebil, Syrine; Korri-Youssoufi, Hafsa; Hunor, Santha; Ohlander, Anna; Kuphal, Mathias; Samitier Marti, Josep.

In: Biosensors and Bioelectronics, Vol. 129, 15.03.2019, p. 147-154.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Samitier Marti, Josep

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