Influence of a protein hydrolysate from green algae on the activity of some ATPase systems in frog skeletal muscle

The present study investigated the effect of a protein hydrolysate from green algae cultured in the Bulgarian region of Rupy, on the enzyme activity of frog skeletal muscle. The activity of pure Mg²⁺-ATPase, Mg²⁺,Ca²⁺- ATPase, NaHCO₃-stimulated Mg²⁺-ATPase and the latter in the presence of the inhibitors NaSCN and NaN₃ in mitochondrial (B-3) and membrane (B-12) fractions were determined before and after treatment with the protein hydrolysate from green algae (30 and 300 μg/ml). The differences between ATPase activity of mitochondrial and membrane fractions were described and it was established that in the B-3 fraction, the activity of the NaHCO₃ stimulated Mg²⁺-ATPase and Ca²⁺-dependent Mg²⁺-ATPase were accelerated by increasing concentrations of the algae protein hydrolysate. Irrespective of the different (equal or inverse) dose-dependent effects, the protein hydrolysate stimulated Mg²⁺-ATPase and that inhibited by NaSCN an NaN₃ bicarbonate-stimulated Mg²⁺-ATPase activity. In most of the probes, the protein hydrolysate produced some increase in enzyme activity of NaHCO₃- stimulated Mg²⁺-ATPase and Ca²⁺-dependent Mg²⁺-ATPase in B-12 fractions. The observed properties of the algae protein hydrolysate suggest that it is capable of stimulating enzyme processes in addition to having some antitoxic effect in skeletal muscle.