Insights into bacterial associations catabolizing atrazine by culture-dependent and molecular approaches

Theresia Komang Ralebitso-Senior, A. Yamazoe, Wilfred F M Roling, Martin Braster, Eric Senior, Henk W van Verseveld

Research output: Contribution to journalArticleResearchpeer-review

Abstract

With the specific selection pressures of four atrazine concentrations (10–33 mg l–1) and two pH values (5.5 and 7.5), eight atrazine-catabolizing microbial associations were enriched and isolated from pesticide-contaminated South African loamy soil. Community-level physiological profiling of Environmental Biolog analysis data identified species complement differences in response to both pH and atrazine concentration and these were confirmed by polymerase chain reaction-based denaturing gradient gel electrophoresis (PCR-DGGE). These differences were not detected by conventional plate cultures and light and scanning electron microscopy. To probe atrazine catabolism under a range of environmental conditions, the two (pH 5.5, KRA02; pH 7.5, KRA06) associations catabolizing 30 mg atrazine l–1 were combined (KRA30). The highest specific growth rate was recorded at pH 4, while at pH 8 little growth resulted. With pH 4-poised cultures, the specific growth rates at 15 and 20 °C were comparable but more than doubled for the next 10° increment. These differences reflected species complement changes. Direct comparison of KRA30 with a reference strain, Pseudomonassp. strain ADP, identified comparable specific growth and atrazine catabolic rates. To probe catabolism further, nitrogen-limited batch cultures were made in the presence of supplemental carbon (citrate) but the catabolic rate did not change. The results are discussed with reference to in situ bioaugmentation remediation programmes.
Original languageEnglish
Pages (from-to)59-67
JournalWorld Journal of Microbiology and Biotechnology
Volume19
Issue number1
DOIs
Publication statusPublished - 2003

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Atrazine
Growth
Denaturing Gradient Gel Electrophoresis
Batch Cell Culture Techniques
Pesticides
Citric Acid
Electron Scanning Microscopy
Adenosine Diphosphate
Nitrogen
Soil
Carbon
Light
Pressure
Polymerase Chain Reaction

Cite this

Ralebitso-Senior, T. K., Yamazoe, A., Roling, W. F. M., Braster, M., Senior, E., & van Verseveld, H. W. (2003). Insights into bacterial associations catabolizing atrazine by culture-dependent and molecular approaches. World Journal of Microbiology and Biotechnology, 19(1), 59-67. https://doi.org/10.1023/A:1022531919239
Ralebitso-Senior, Theresia Komang ; Yamazoe, A. ; Roling, Wilfred F M ; Braster, Martin ; Senior, Eric ; van Verseveld, Henk W. / Insights into bacterial associations catabolizing atrazine by culture-dependent and molecular approaches. In: World Journal of Microbiology and Biotechnology. 2003 ; Vol. 19, No. 1. pp. 59-67.
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Ralebitso-Senior, TK, Yamazoe, A, Roling, WFM, Braster, M, Senior, E & van Verseveld, HW 2003, 'Insights into bacterial associations catabolizing atrazine by culture-dependent and molecular approaches', World Journal of Microbiology and Biotechnology, vol. 19, no. 1, pp. 59-67. https://doi.org/10.1023/A:1022531919239

Insights into bacterial associations catabolizing atrazine by culture-dependent and molecular approaches. / Ralebitso-Senior, Theresia Komang; Yamazoe, A.; Roling, Wilfred F M; Braster, Martin; Senior, Eric; van Verseveld, Henk W.

In: World Journal of Microbiology and Biotechnology, Vol. 19, No. 1, 2003, p. 59-67.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Ralebitso-Senior, Theresia Komang

AU - Yamazoe, A.

AU - Roling, Wilfred F M

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AU - Senior, Eric

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AB - With the specific selection pressures of four atrazine concentrations (10–33 mg l–1) and two pH values (5.5 and 7.5), eight atrazine-catabolizing microbial associations were enriched and isolated from pesticide-contaminated South African loamy soil. Community-level physiological profiling of Environmental Biolog analysis data identified species complement differences in response to both pH and atrazine concentration and these were confirmed by polymerase chain reaction-based denaturing gradient gel electrophoresis (PCR-DGGE). These differences were not detected by conventional plate cultures and light and scanning electron microscopy. To probe atrazine catabolism under a range of environmental conditions, the two (pH 5.5, KRA02; pH 7.5, KRA06) associations catabolizing 30 mg atrazine l–1 were combined (KRA30). The highest specific growth rate was recorded at pH 4, while at pH 8 little growth resulted. With pH 4-poised cultures, the specific growth rates at 15 and 20 °C were comparable but more than doubled for the next 10° increment. These differences reflected species complement changes. Direct comparison of KRA30 with a reference strain, Pseudomonassp. strain ADP, identified comparable specific growth and atrazine catabolic rates. To probe catabolism further, nitrogen-limited batch cultures were made in the presence of supplemental carbon (citrate) but the catabolic rate did not change. The results are discussed with reference to in situ bioaugmentation remediation programmes.

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