Introduction: It is increasingly becoming a requirement for radiographers to use the concept of Evidence Based Practice (EBP) to inform their clinical practice. The purpose of this study was to assess knowledge, attitudes and practices towards the use of EBP as well as to establish factors that influence the use of EBP amongst radiographers in Uganda. Methods: A total of 83 respondents participated in a cross-sectional survey conducted amongst qualified radiographers licensed to practice in Uganda. Data was collected using a self-reporting questionnaire that was accessed through the Bristol Online Survey Software. Using SPSS version 21, descriptive and inferential statistics were generated. Results: The study revealed that 47 (57%) and 36 (43%) respondents scored themselves as having high and low knowledge of EBP respectively. However, 52 (63%) respondents scored themselves as having a negative attitude towards EBP. As regards use of EBP, 49 (59%) out of 83 respondents reported use of EBP in clinical practice. Using binary logistic regression, a significant association was found between a high knowledge level and the use of EBP (Adjusted OR, 95% CI: 9.89: 3.54–27.64). Conclusion: Overall, majority of the respondents had high knowledge levels about EBP. However, a high proportion of them were found to have a negative attitude towards EBP. High knowledge levels of EBP were strongly associated with use of EBP in practice. This finding provides helpful insights for policy makers to consider a holistic approach towards improving the use of EBP among radiographers through devising interventions that strengthen both knowledge and attitude towards EBP.