Label-free bacteria quantification in blood plasma by a bioprinted microarray based interferometric point-of-care device

Priyanka Dey, Nuria Fabri-Faja, Olalla Calvo-Lozano, Roland A. Terborg, Alexander Belushkin, Filiz Yesilkoy, Anna Fàbrega, Juan Carlos Ruiz-Rodriguez, Ricard Ferrer, Juan José González-López, Maria Carmen Estévez, Hatice Altug, Valerio Pruneri, Laura M. Lechuga

Research output: Contribution to journalArticlepeer-review

Abstract

Existing clinical methods for bacteria detection lack speed, sensitivity, and, importantly, point-of-care (PoC) applicability. Thus, finding ways to push the sensitivity of clinical PoC biosensing technologies is crucial. Here we report a portable PoC device based on lens-free interferometric microscopy (LIM). The device employs high performance nanoplasmonics and custom bioprinted microarrays and is capable of direct label-free bacteria (E. coli) quantification. With only one-step sample handling we offer a sample-to-data turnaround time of 40 min. Our technology features detection sensitivity of a single bacterial cell both in buffer and in diluted blood plasma and is intrinsically limited by the number of cells present in the detection volume. When employed in a hospital setting, the device has enabled accurate categorization of sepsis patients (infectious SIRS) from control groups (healthy individuals and noninfectious SIRS patients) without false positives/negatives. User-friendly on-site bacterial clinical diagnosis can thus become a reality.
Original languageEnglish
Pages (from-to)52–60
JournalACS Sensors
Volume4
Issue number1
DOIs
Publication statusPublished - 25 Jan 2019

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