Methods of synchronization of yeast cells for the analysis of cell cycle progression

    Research output: Chapter in Book/Report/Conference proceedingChapter

    3 Citations (Scopus)

    Abstract

    Cell division is a fascinating and fundamental process that sustains life. By this process, unicellular organisms reproduce and multicellular organisms sustain development, growth, and tissue repair. Division of a mother cell gives rise to two daughter cells according to an ordered set of events within four successive phases called G1 (gap1), S (DNA Synthesis), G2 (gap2), and M (Afitosis) phase. How these different phases are orchestrated to ensure the physical separation of the two daughter cells is a tightly regulated process. Indeed, inappropriate cell division could lead to uncontrolled cell proliferation and ultimately to cancer. Saccharomyces cerevisiae is an excellent model system for unraveling the secrets of cell division. A large community of researchers has chosen budding yeast as a model because of its advantages: rapid growth in simple and economical media, tractable genetics, powerful biochemistry, cell biology, and pro-teomics approaches. Furthermore, the cell cycle mechanisms, as elucidated in yeast, are conserved in higher eukaryotes. The ability to synchronize and get large numbers of cells in a particular stage of the cell cycle is crucial to properly explore the mechanisms of the cell cycle. An overview of the most common yeast synchronization techniques has been compiled in this chapter.

    Original languageEnglish
    Title of host publicationThe Mitotic Exit network
    PublisherHumana Press Inc.
    Pages19-34
    Number of pages16
    DOIs
    Publication statusPublished - 1 Jan 2017

    Publication series

    NameMethods in Molecular Biology
    Volume1505
    ISSN (Print)1064-3745

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  • Cite this

    Angeles Juanes, M. (2017). Methods of synchronization of yeast cells for the analysis of cell cycle progression. In The Mitotic Exit network (pp. 19-34). (Methods in Molecular Biology; Vol. 1505). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6502-1_2