Microbial analysis using Sharma's reaction

Zulfiqur Ali; Bruce Hodson; James Timmins; Stephen Keith

doi:10.1117/12.339046

Microbial analysis using Sharma's reaction

Zulfiqur Ali, Bruce Hodson, James Timmins, Stephen Keith

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The photoreaction of thionine with NAD(P)H leads to the formation of non-fluorescent semi and lecuothionine. This has been previously used for the development of enzymatic assays. We show that the fluorescence of thionine is quenched in the presence of Nocardia corallina. We ascribe this decrease in fluorescence to the presence of NAD(P)H present on the walls and in the body of the cells as described by Sharma. We therefore demonstrate that it is possible to measure, in situ, NAD(P)H in Nocardia corallina in a simple and inexpensive manner and that this finding extends the analytical application of Sharma's reaction to other organisms.

Original language	English
Title of host publication	Environmental monitoring and remediation technologies
Editors	Tuan Vo-Dinh, Robert L Spellicy
Publisher	SPIE Society of Photo-Optical Instrumentation Engineers
Pages	641-646
DOIs	https://doi.org/10.1117/12.339046
Publication status	Published - Feb 1999
Event	1998 Environmental Monitoring and Remediation Technologies - Boston, United States Duration: 2 Nov 1998 → 5 Nov 1998

Conference

Conference	1998 Environmental Monitoring and Remediation Technologies
Country/Territory	United States
City	Boston
Period	2/11/98 → 5/11/98

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10.1117/12.339046

http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=968350

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@inproceedings{fd5f726898ff4abea8fbe08a1f2fc94a,

title = "Microbial analysis using Sharma's reaction",

abstract = "The photoreaction of thionine with NAD(P)H leads to the formation of non-fluorescent semi and lecuothionine. This has been previously used for the development of enzymatic assays. We show that the fluorescence of thionine is quenched in the presence of Nocardia corallina. We ascribe this decrease in fluorescence to the presence of NAD(P)H present on the walls and in the body of the cells as described by Sharma. We therefore demonstrate that it is possible to measure, in situ, NAD(P)H in Nocardia corallina in a simple and inexpensive manner and that this finding extends the analytical application of Sharma's reaction to other organisms.",

author = "Zulfiqur Ali and Bruce Hodson and James Timmins and Stephen Keith",

year = "1999",

month = feb,

doi = "10.1117/12.339046",

language = "English",

pages = "641--646",

editor = "Tuan Vo-Dinh and Spellicy, {Robert L}",

booktitle = "Environmental monitoring and remediation technologies",

publisher = "SPIE Society of Photo-Optical Instrumentation Engineers ",

note = "1998 Environmental Monitoring and Remediation Technologies ; Conference date: 02-11-1998 Through 05-11-1998",

}

Ali, Z, Hodson, B, Timmins, J & Keith, S 1999, Microbial analysis using Sharma's reaction. in T Vo-Dinh & RL Spellicy (eds), Environmental monitoring and remediation technologies. SPIE Society of Photo-Optical Instrumentation Engineers , pp. 641-646, 1998 Environmental Monitoring and Remediation Technologies, Boston, United States, 2/11/98. https://doi.org/10.1117/12.339046

TY - GEN

T1 - Microbial analysis using Sharma's reaction

AU - Ali, Zulfiqur

AU - Hodson, Bruce

AU - Timmins, James

AU - Keith, Stephen

PY - 1999/2

Y1 - 1999/2

N2 - The photoreaction of thionine with NAD(P)H leads to the formation of non-fluorescent semi and lecuothionine. This has been previously used for the development of enzymatic assays. We show that the fluorescence of thionine is quenched in the presence of Nocardia corallina. We ascribe this decrease in fluorescence to the presence of NAD(P)H present on the walls and in the body of the cells as described by Sharma. We therefore demonstrate that it is possible to measure, in situ, NAD(P)H in Nocardia corallina in a simple and inexpensive manner and that this finding extends the analytical application of Sharma's reaction to other organisms.

AB - The photoreaction of thionine with NAD(P)H leads to the formation of non-fluorescent semi and lecuothionine. This has been previously used for the development of enzymatic assays. We show that the fluorescence of thionine is quenched in the presence of Nocardia corallina. We ascribe this decrease in fluorescence to the presence of NAD(P)H present on the walls and in the body of the cells as described by Sharma. We therefore demonstrate that it is possible to measure, in situ, NAD(P)H in Nocardia corallina in a simple and inexpensive manner and that this finding extends the analytical application of Sharma's reaction to other organisms.

U2 - 10.1117/12.339046

DO - 10.1117/12.339046

M3 - Conference contribution

SP - 641

EP - 646

BT - Environmental monitoring and remediation technologies

A2 - Vo-Dinh, Tuan

A2 - Spellicy, Robert L

PB - SPIE Society of Photo-Optical Instrumentation Engineers

T2 - 1998 Environmental Monitoring and Remediation Technologies

Y2 - 2 November 1998 through 5 November 1998

ER -

Microbial analysis using Sharma's reaction

Abstract

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