Microbial analysis using Sharma's reaction

Zulfiqur Ali, Bruce Hodson, James Timmins, Stephen Keith

    Research output: Chapter in Book/Report/Conference proceedingConference contribution


    The photoreaction of thionine with NAD(P)H leads to the formation of non-fluorescent semi and lecuothionine. This has been previously used for the development of enzymatic assays. We show that the fluorescence of thionine is quenched in the presence of Nocardia corallina. We ascribe this decrease in fluorescence to the presence of NAD(P)H present on the walls and in the body of the cells as described by Sharma. We therefore demonstrate that it is possible to measure, in situ, NAD(P)H in Nocardia corallina in a simple and inexpensive manner and that this finding extends the analytical application of Sharma's reaction to other organisms.
    Original languageEnglish
    Title of host publicationEnvironmental monitoring and remediation technologies
    EditorsTuan Vo-Dinh, Robert L Spellicy
    PublisherSPIE Society of Photo-Optical Instrumentation Engineers
    Publication statusPublished - Feb 1999
    Event1998 Environmental Monitoring and Remediation Technologies - Boston, United States
    Duration: 2 Nov 19985 Nov 1998


    Conference1998 Environmental Monitoring and Remediation Technologies
    Country/TerritoryUnited States


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