Abstract
A prototype Polydimethylsiloxane (PDMS) based microbioreactor integrated with a photodiode detector for monitoring intracellular green fluorescent protein ultraviolet (GFPUV) expression is described. The developed system is compact, simple and inexpensive thus making it ideal for an economical on chip detection of intracellular GFP and other biological applications. The detection limit for cell free GFPUV was found to be 4.8 × 10-8 M while for Fluorescein isothiocyanate (FITC) was found to be 1.2 × 10-6 M. The performance of the photodiode detector was benchmarked with a CCD spectrophotometer and results showed favorable comparison. This study demonstrates the quest to develop integrated microsystems that can be used to monitor cellular dynamics in real time.
Original language | English |
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Title of host publication | 13th Mediterranean Conference on Medical and Biological Engineering and Computing 2013 - MEDICON 2013 |
Publisher | Springer Verlag |
Pages | 888-891 |
Number of pages | 4 |
Volume | 41 |
ISBN (Print) | 9783319008455 |
DOIs | |
Publication status | Published - 1 Jan 2014 |
Event | 13th Mediterranean Conference on Medical and Biological Engineering and Computing - Seville, Spain Duration: 25 Sept 2013 → 28 Sept 2013 |
Conference
Conference | 13th Mediterranean Conference on Medical and Biological Engineering and Computing |
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Abbreviated title | MEDICON 2013 |
Country/Territory | Spain |
City | Seville |
Period | 25/09/13 → 28/09/13 |