Microbioreactor integrated with a sensor for monitoring intracellular green fluorescence protein (GFP)

Godfrey Pasirayi, Meez Islam, Simon Scott, Liam O'Hare, Zulfiqur Ali

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearch

1 Citation (Scopus)

Abstract

A prototype Polydimethylsiloxane (PDMS) based microbioreactor integrated with a photodiode detector for monitoring intracellular green fluorescent protein ultraviolet (GFPUV) expression is described. The developed system is compact, simple and inexpensive thus making it ideal for an economical on chip detection of intracellular GFP and other biological applications. The detection limit for cell free GFPUV was found to be 4.8 × 10-8 M while for Fluorescein isothiocyanate (FITC) was found to be 1.2 × 10-6 M. The performance of the photodiode detector was benchmarked with a CCD spectrophotometer and results showed favorable comparison. This study demonstrates the quest to develop integrated microsystems that can be used to monitor cellular dynamics in real time.

Original languageEnglish
Title of host publication13th Mediterranean Conference on Medical and Biological Engineering and Computing 2013 - MEDICON 2013
PublisherSpringer Verlag
Pages888-891
Number of pages4
Volume41
ISBN (Print)9783319008455
DOIs
Publication statusPublished - 1 Jan 2014
Event13th Mediterranean Conference on Medical and Biological Engineering and Computing - Seville, Spain
Duration: 25 Sep 201328 Sep 2013

Conference

Conference13th Mediterranean Conference on Medical and Biological Engineering and Computing
Abbreviated titleMEDICON 2013
CountrySpain
CitySeville
Period25/09/1328/09/13

Fingerprint

Green Fluorescent Proteins
Photodiodes
Fluorescence
Detectors
Monitoring
Microsystems
Spectrophotometers
Sensors
Fluorescein
Charge coupled devices
Proteins
baysilon
isothiocyanic acid

Cite this

Pasirayi, G., Islam, M., Scott, S., O'Hare, L., & Ali, Z. (2014). Microbioreactor integrated with a sensor for monitoring intracellular green fluorescence protein (GFP). In 13th Mediterranean Conference on Medical and Biological Engineering and Computing 2013 - MEDICON 2013 (Vol. 41, pp. 888-891). Springer Verlag. https://doi.org/10.1007/978-3-319-00846-2_220
Pasirayi, Godfrey ; Islam, Meez ; Scott, Simon ; O'Hare, Liam ; Ali, Zulfiqur. / Microbioreactor integrated with a sensor for monitoring intracellular green fluorescence protein (GFP). 13th Mediterranean Conference on Medical and Biological Engineering and Computing 2013 - MEDICON 2013. Vol. 41 Springer Verlag, 2014. pp. 888-891
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Pasirayi, G, Islam, M, Scott, S, O'Hare, L & Ali, Z 2014, Microbioreactor integrated with a sensor for monitoring intracellular green fluorescence protein (GFP). in 13th Mediterranean Conference on Medical and Biological Engineering and Computing 2013 - MEDICON 2013. vol. 41, Springer Verlag, pp. 888-891, 13th Mediterranean Conference on Medical and Biological Engineering and Computing, Seville, Spain, 25/09/13. https://doi.org/10.1007/978-3-319-00846-2_220

Microbioreactor integrated with a sensor for monitoring intracellular green fluorescence protein (GFP). / Pasirayi, Godfrey; Islam, Meez; Scott, Simon ; O'Hare, Liam; Ali, Zulfiqur.

13th Mediterranean Conference on Medical and Biological Engineering and Computing 2013 - MEDICON 2013. Vol. 41 Springer Verlag, 2014. p. 888-891.

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearch

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Pasirayi G, Islam M, Scott S, O'Hare L, Ali Z. Microbioreactor integrated with a sensor for monitoring intracellular green fluorescence protein (GFP). In 13th Mediterranean Conference on Medical and Biological Engineering and Computing 2013 - MEDICON 2013. Vol. 41. Springer Verlag. 2014. p. 888-891 https://doi.org/10.1007/978-3-319-00846-2_220