TY - JOUR
T1 - Microfluidic Bioreactors for Cell Culturing: A Review
AU - Pasirayi, Godfrey
AU - Auger, Vincent
AU - Scott, Simon M.
AU - Rahman, Pattanathu K.S.M.
AU - Islam, Meez
AU - O'Hare, Liam
AU - Ali, Zulfiqur
N1 - Authors can self-archive and post their articles on any digital repository. [Email from Bentham Science Publishers]
PY - 2011/7/10
Y1 - 2011/7/10
N2 - The use of microfluidic bioreactor platforms for cell culturing holds considerable promise for a range of fields which include drug discovery, tissue engineering, bioprocessing optimisation and cell based screening studies. Microfluidic bioreactor systems have length scales that are well matched to the physical dimensions of most cells and microorganisms. In view of this, microfluidic bioreactors have attractive features which make them ideal to study the behaviour of cells and their internal organisation in their native microenvironment. Due to their small footprint microbioreactor platforms offer a number of advantages over conventional macroscale systems including improved biological function, higher quality cell-based data, reduced volume of reagents, ease of integration and lower cost. This review highlights the basic concepts, designs and operational requirements of microbioreactors for cell based studies. An illustrative outline of different applications of microbioreactors and some indication of new trends and progress in recent years are provided. Specific examples of applications of microbioreactors are drawn for cytotoxicity assays, tissue engineering, stem cells, microbialfermentations, single cell analysis and in vitro fertilisation.
AB - The use of microfluidic bioreactor platforms for cell culturing holds considerable promise for a range of fields which include drug discovery, tissue engineering, bioprocessing optimisation and cell based screening studies. Microfluidic bioreactor systems have length scales that are well matched to the physical dimensions of most cells and microorganisms. In view of this, microfluidic bioreactors have attractive features which make them ideal to study the behaviour of cells and their internal organisation in their native microenvironment. Due to their small footprint microbioreactor platforms offer a number of advantages over conventional macroscale systems including improved biological function, higher quality cell-based data, reduced volume of reagents, ease of integration and lower cost. This review highlights the basic concepts, designs and operational requirements of microbioreactors for cell based studies. An illustrative outline of different applications of microbioreactors and some indication of new trends and progress in recent years are provided. Specific examples of applications of microbioreactors are drawn for cytotoxicity assays, tissue engineering, stem cells, microbialfermentations, single cell analysis and in vitro fertilisation.
U2 - 10.2174/1876402911103020137
DO - 10.2174/1876402911103020137
M3 - Article
SN - 1876-4029
VL - 3
SP - 137
EP - 160
JO - Micro and Nanosystems
JF - Micro and Nanosystems
IS - 2
ER -