TY - JOUR
T1 - Modulation of human gut microbiota composition and metabolites by arabinogalactan and Bifidobacterium longum subsp. longum BB536 in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®)
AU - Wang, Yan
AU - Liu, Yafei
AU - Polic, Ives Ivusic
AU - Matheyambath, Ajila Chandran
AU - LaPointe, Gisèle
PY - 2021
Y1 - 2021
N2 - The Mucosal-Simulator of the Human Intestinal Microbial Ecosystem (SHIME) was used to differentiate the gut microbial fermentation of arabinogalactan (AG) and starch before (T1), during (T2), and after (T3) one-week Bifidobacterium longum subsp. longum BB536 supplementation. Adding 10 g/L of either AG or starch to the basal medium (T1) promoted short-chain fatty acid (SCFA) production in three successive SHIME colon vessels. AG fermentation (T1) resulted in higher total SCFAs (P < 0.05) and luminal live Faecalibacterium prausnitzii (P < 0.0001) in the transverse colon than starch fermentation (T1). Compared to AG fermentation alone (T1), B. longum supplementation (T2) significantly enhanced butyrate production (P < 0.05) and the abundance of luminal live F. prausnitzii (P < 0.0001) in the transverse colon. The results indicate that combining a potential prebiotic and a probiotic strain increases SCFA production and particular gut commensals which could have a beneficial effect on gut health.
AB - The Mucosal-Simulator of the Human Intestinal Microbial Ecosystem (SHIME) was used to differentiate the gut microbial fermentation of arabinogalactan (AG) and starch before (T1), during (T2), and after (T3) one-week Bifidobacterium longum subsp. longum BB536 supplementation. Adding 10 g/L of either AG or starch to the basal medium (T1) promoted short-chain fatty acid (SCFA) production in three successive SHIME colon vessels. AG fermentation (T1) resulted in higher total SCFAs (P < 0.05) and luminal live Faecalibacterium prausnitzii (P < 0.0001) in the transverse colon than starch fermentation (T1). Compared to AG fermentation alone (T1), B. longum supplementation (T2) significantly enhanced butyrate production (P < 0.05) and the abundance of luminal live F. prausnitzii (P < 0.0001) in the transverse colon. The results indicate that combining a potential prebiotic and a probiotic strain increases SCFA production and particular gut commensals which could have a beneficial effect on gut health.
U2 - 10.1016/j.jff.2021.104820
DO - 10.1016/j.jff.2021.104820
M3 - Article
SN - 1756-4646
VL - 87
JO - Journal of Functional Foods
JF - Journal of Functional Foods
M1 - 104820
ER -